| 顾源,汤新星,张圆圆,姚雪婷,王畅.电离辐射致大鼠肠上皮细胞磷脂变化的研究[J].中华放射医学与防护杂志,2013,33(5):457-462 |
| 电离辐射致大鼠肠上皮细胞磷脂变化的研究 |
| Alternations of phospholipids in the rat intestinal epithelial cells after ionizing radiation |
| 投稿时间：2013-01-04 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.001 |
| 中文关键词: 电离辐射 小肠上皮细胞 磷脂 |
| 英文关键词:Ionizing radiation Intestinal epithelial cells Phospholipid |
| 基金项目:国家自然科学基金(31000385);江苏省高校自然科学研究项目(10KJB310012);江苏省博士后基金(0901052C);大学生创新创业训练计划(2012yd016) |
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| 中文摘要: |
| 目的 观察电离辐射所致肠上皮细胞磷脂的变化,探讨放射性肠损伤的发生机制。方法 将大鼠小肠上皮细胞(IEC-6)分为3组:正常对照组、8 Gy X射线照射组和12 Gy X射线照射组。分别在照射后6和24 h,提取IEC-6细胞磷脂,利用液相色谱-质谱联用技术(HPLC-MS)测定辐射所致细胞磷脂的变化。结果 辐射后6 h,8 Gy照射组细胞磷脂未发生显著变化;12 Gy照射组细胞磷脂变化明显,其中磷脂酰甘油(PG)、磷脂酰肌醇(PI)和溶血磷脂酰胆碱(Lyso PC)均显著性上调(F=5.37、9.60、9.88,P<0.05)。而照射后24 h,两个辐射剂量组中多种磷脂酰乙醇胺(PE)和PG分子显著下调(F=5.15~99.77,P<0.05),12 Gy照射组中多种神经鞘磷脂(SM)显著上调(F=4.35~7.92,P<0.05)。结论 电离辐射可导致大鼠肠上皮细胞(IEC-6)磷脂代谢紊乱,其紊乱程度与辐射剂量相关。 |
| 英文摘要: |
| Objective To investigate radiation-induced alternations of phospholipids in epithelial cells, and to provide experimental evidence for understanding the mechanism of radiation-induced intestinal injury. Methods The intestinal epithelial cells (IEC-6) in rats were divided into three groups: normal control group, 8 Gy X-ray irradiation group and 12 Gy X-ray irradiation group. Phospholipids were extracted at 6 h or 24 h after radiation and then measured by high-performance liquid chromatography and mass spectrometry (HPLC-MS). Results At 6 h after radiation, the phospholipids in 8 Gy irradiation group didn't vary significantly, while those in 12 Gy irradiation group changed. The PG, PI and Lyso PC were significantly up-regulated (F=5.37, 9.60, 9.88, P<0.05). However, at 24 h after radiation, many PE and PG species in both irradiation groups declined (F=5.15-99.77, P<0.05) and SM species increased in 12 Gy irradiation group (F=4.35-7.92, P<0.05). Conclusions The ionizing radiation could disorder phospholipid metabolism in IEC-6 cells with a dose-dependent manner. |
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