| 刘澜涛,刘建香,苏旭.2Gy60Coγ射线照射诱导调节性T细胞的辐射凋亡机制[J].中华放射医学与防护杂志,2013,33(4):364-367 |
| 2Gy60Coγ射线照射诱导调节性T细胞的辐射凋亡机制 |
| Mechanism of 2 Gy 60Co γ-ray-induced apoptosis in the regulatory T cells of mice |
| 投稿时间：2013-01-25 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.04.007 |
| 中文关键词: 电离辐射 调节性T细胞 凋亡 CD39 |
| 英文关键词:Ionizing radiation Regulatory T cell Apoptosis CD39 |
| 基金项目:卫生行业科研专项(201002009) |
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| 中文摘要: |
| 目的 分析2 Gy 60Co γ射线照射对小鼠调节性T细胞存活率的影响,并初步探讨Treg细胞凋亡相关因子Bcl-2、Bax与CD39表达之间的关系。方法 用2 Gy 60Co γ射线全身照射小鼠,分离胸腺、脾脏中的淋巴细胞,计数细胞数后利用流式细胞仪对Treg细胞亚群的凋亡率、CD39及凋亡相关因子的表达进行分析。结果 受照后Treg细胞较CD4+FOXP3-T细胞的存活率高,胸腺和脾脏中Treg细胞存活率的时间效应关系不同;受照后Treg细胞凋亡率增加,以照后4 d增幅最为显著(t=-3.6、-7.4, P<0.05);受照后胸腺与脾脏中Bax/Bcl-2的比值变化规律不同,前者中该比值增加,以照后4 d增加最为显著(t=-3.4, P<0.05),而脾脏中该比值于照后4 d减小(t=2.4, P<0.05);受照后4 d胸腺Treg细胞中CD39表达上调(t=-6.6, P<0.05),而脾脏中表达下调(t=2.6, P<0.05)。结论 受照后Treg细胞凋亡率、Bax/Bcl-2比值的变化规律与CD39的表达规律基本一致。 |
| 英文摘要: |
| Objective To analyze the influence of ionizing radiation on the viability of the regulatory T cells and the relationship between the expression of CD39 and apoptosis related factors in mice Treg cells. Methods Mice were administered to whole body irradiation with 2 Gy of γ-rays, then lymphocytes were separated from thymus and spleen. The percentages of CD4+ T and CD4+ FOXP3+ CD25+ Treg lymphocytes, apoptosis related genes of Bax,Bcl-2 and CD39 were analyzed with FACS. Results Treg cells had a higher survival fraction than CD4+FOXP3-Tcon cells after irradiation, and the time-responses of Treg cells in thymus and spleen were different. The apoptosis rate of Treg cells increased at 4 d after irradiation(t=-3.6,-7.4, P<0.05). The Bax/Bcl-2 ratio and the CD39 expression were increased in thymus Treg cells (t=-3.4,-6.6, P<0.05), but decreased in spleen Treg cells (t=2.4, 2.6, P<0.05). Conclusions The changes of apoptosis and Bax/Bcl-2 are in consistent with the expression of CD39 in the irradiated Treg cells. |
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