中华放射医学与防护杂志

刘澜涛,刘建香,苏旭.2Gy⁶⁰Coγ射线照射诱导调节性T细胞的辐射凋亡机制[J].中华放射医学与防护杂志,2013,33(4):364-367.LIU Lan-tao,LIU Jian-xiang,SU Xu.Mechanism of 2 Gy ⁶⁰Co γ-ray-induced apoptosis in the regulatory T cells of mice[J].Chin J Radiol Med Prot,2013,33(4):364-367

2Gy⁶⁰Coγ射线照射诱导调节性T细胞的辐射凋亡机制

Mechanism of 2 Gy ⁶⁰Co γ-ray-induced apoptosis in the regulatory T cells of mice

投稿时间：2013-01-25

DOI：10.3760/cma.j.issn.0254-5098.2013.04.007

中文关键词: 电离辐射调节性T细胞凋亡 CD39

英文关键词:Ionizing radiation Regulatory T cell Apoptosis CD39

基金项目:卫生行业科研专项(201002009)

作者	单位	E-mail
刘澜涛	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室
刘建香	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室
苏旭	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室	suxu@nirp.cn

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中文摘要:

目的分析2 Gy ⁶⁰Co γ射线照射对小鼠调节性T细胞存活率的影响,并初步探讨Treg细胞凋亡相关因子Bcl-2、Bax与CD39表达之间的关系。方法用2 Gy ⁶⁰Co γ射线全身照射小鼠,分离胸腺、脾脏中的淋巴细胞,计数细胞数后利用流式细胞仪对Treg细胞亚群的凋亡率、CD39及凋亡相关因子的表达进行分析。结果受照后Treg细胞较CD4+FOXP3-T细胞的存活率高,胸腺和脾脏中Treg细胞存活率的时间效应关系不同;受照后Treg细胞凋亡率增加,以照后4 d增幅最为显著(t=-3.6、-7.4, P<0.05);受照后胸腺与脾脏中Bax/Bcl-2的比值变化规律不同,前者中该比值增加,以照后4 d增加最为显著(t=-3.4, P<0.05),而脾脏中该比值于照后4 d减小(t=2.4, P<0.05);受照后4 d胸腺Treg细胞中CD39表达上调(t=-6.6, P<0.05),而脾脏中表达下调(t=2.6, P<0.05)。结论受照后Treg细胞凋亡率、Bax/Bcl-2比值的变化规律与CD39的表达规律基本一致。

英文摘要:

Objective To analyze the influence of ionizing radiation on the viability of the regulatory T cells and the relationship between the expression of CD39 and apoptosis related factors in mice Treg cells. Methods Mice were administered to whole body irradiation with 2 Gy of γ-rays, then lymphocytes were separated from thymus and spleen. The percentages of CD4+ T and CD4+ FOXP3+ CD25+ Treg lymphocytes, apoptosis related genes of Bax,Bcl-2 and CD39 were analyzed with FACS. Results Treg cells had a higher survival fraction than CD4+FOXP3-Tcon cells after irradiation, and the time-responses of Treg cells in thymus and spleen were different. The apoptosis rate of Treg cells increased at 4 d after irradiation(t=-3.6,-7.4, P<0.05). The Bax/Bcl-2 ratio and the CD39 expression were increased in thymus Treg cells (t=-3.4,-6.6, P<0.05), but decreased in spleen Treg cells (t=2.4, 2.6, P<0.05). Conclusions The changes of apoptosis and Bax/Bcl-2 are in consistent with the expression of CD39 in the irradiated Treg cells.

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