| 白明华,张扬,王宝峰,等.姜黄素提高人胰腺癌PANC-1细胞放射敏感性的机制研究[J].中华放射医学与防护杂志,2013,33(4):360-363.BAI Ming-hua,ZHANG Yang,WANG Bao-feng,et al.Mechanism of curcumin-enhanced radiosensitivity in pancreatic cancer cells PANC-1[J].Chin J Radiol Med Prot,2013,33(4):360-363 |
| 姜黄素提高人胰腺癌PANC-1细胞放射敏感性的机制研究 |
| Mechanism of curcumin-enhanced radiosensitivity in pancreatic cancer cells PANC-1 |
| 投稿时间：2013-01-11 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.04.006 |
| 中文关键词: 姜黄素 γ射线 PANC-1细胞 P21 胰腺癌 |
| 英文关键词:Curcumin γ-rays PANC-1 cells P21 Pancreatic cancer |
| 基金项目:西安市科技计划医学攻关项目(GG06187) |
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| 中文摘要: |
| 目的 观察姜黄素对人胰腺癌PANC-1细胞放射敏感性的影响。方法 γ射线照射人胰腺癌PANC-1细胞,同时联合应用姜黄素处理细胞,通过MTT法检测PANC-1细胞增殖活性的变化,利用流式细胞仪观察PANC-1细胞周期和凋亡率的变化,采用RT-PCR和Western blot分析PANC-1细胞中P21表达的变化。结果 与γ射线照射组比较,γ射线联合应用姜黄素可以显著降低PANC-1细胞的增殖活性(t=6.72, P<0.01),同时影响细胞周期的变化,显著增加S期细胞(t=4.78, P<0.05),PANC-1细胞凋亡明显提高(t=6.58,P<0.01),并伴有P21蛋白和mRNA表达的变化(t=5.72、5.63, P<0.01)。结论 姜黄素可以显著提高人胰腺癌PANC-1细胞的放疗敏感性,这一特性与P21的表达有关。 |
| 英文摘要: |
| Objective To investigate the combination effect of curcumin and γ-ray irradiation on PANC-1 cells in vitro. Methods PANC-1 cells were exposed to γ-rays in the presence or absence of curcumin. MTT assay was performed to evaluate cell viability. The expression of P21 was evaluated with RT-PCR and Western blot. Cell cycle distribution and apoptosis were tested by flow cytometry. Results Compared with the γ-ray irradiation group, combination treatment of curcumin and irradiation decreased the cell viability (t=6.72, P<0.01) and increased the percentage of cells in S-phase (t=4.78, P<0.05), apoptosis rate (t=6.58,P<0.01), P21 protein and mRNA expression (t=5.72, 5.63, P<0.01) in PANC-1 cells. Conclusions Curcumin increases the radiosensitivity of PANC-1 cells, which may have clinical implication on radiotherapy of pancreatic cancer. |
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