| 孙志强,于静萍,张志明,等.重组人血管内皮抑素对食管癌细胞的放射增敏作用及其机制[J].中华放射医学与防护杂志,2013,33(4):346-350.SUN Zhi-qiang,YU Jing-ping,ZHANG Zhi-ming,et al.Effect of recombinant human endostatin on the radiosensitivity of esophageal carcinoma cells and underlying mechanism[J].Chin J Radiol Med Prot,2013,33(4):346-350 |
| 重组人血管内皮抑素对食管癌细胞的放射增敏作用及其机制 |
| Effect of recombinant human endostatin on the radiosensitivity of esophageal carcinoma cells and underlying mechanism |
| 投稿时间：2012-12-21 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.04.003 |
| 中文关键词: 重组人血管内皮抑素 食管癌细胞 辐射敏感性 |
| 英文关键词:Recombinant human endostatin Esophageal carcinoma cells Radiosensitivity |
| 基金项目:江苏省卫生厅指导性科研项目(Z201220);常州市卫生局重大项目(ZD201105);常州市科技支撑社会发展项目(CE20125021) |
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| 中文摘要: |
| 目的 观察重组人血管内皮抑素(rhES)对食管鳞癌KYSE-150细胞的放射敏感性的影响及其机制的初步探讨。方法 将食管鳞癌KYSE-150细胞分为空白对照组、rhES组、X射线照射组和rhES联合X射线照射组。细胞克隆形成法测定细胞存活分数;单击多靶模型拟合细胞存活曲线并计算放射增敏比(SER);流式细胞术检测rhES联合X射线照射对细胞周期及凋亡的影响;反转录聚合酶链反应(RT-PCR)检测Cyclin B1、Cyclin D1、Bcl-2、Bax mRNA表达水平,Western blot检测HIF-1α、VEGF、VEGFR蛋白的表达水平。结果 KYSE-150细胞的D0、Dq、SF2值随着rhES浓度的增加逐渐减小,当达到D0照射剂量时,100和200 μg/ml rhES的放射增敏比分别为1.14、1.27;rhES联合X射线照射组与X射线照射组比较,细胞凋亡率增加、凋亡相关基因bax的表达增加、细胞VEGF蛋白及HIF-1α蛋白的表达水平降低(t=7.97、3.02、117.55、7.22,P<0.05)。结论 rhES对体外食管癌细胞具有明显的辐射增敏作用,其机制与其抑制细胞HIF-1α、VEGF蛋白表达以及对细胞凋亡相关基因bax的表达调控、诱导细胞凋亡密切相关。 |
| 英文摘要: |
| Objective To study the effect of recombinant human endostatin(rhES)on the radiosensitivity of esophageal squamous cells KYSE-150 and its preliminary mechanism. Methods Cells were divided into four groups: control group without treatment, rhES group treated with recombinant human endostatin, radiation alone group exposed with X-rays, and combination group exposed with X-rays plus endostatin. Colony formation assay was used to measure cell survival fraction. A single-hit multi-target model was used to fit cell survival curve and calculate the sensitive enhancement ratio (SER). Influence of rhES combined with X-ray radiation on cell cycle and apoptosis was measured by flow cytometry. Expressions of Cyclin B1, Cyclin D1, Bcl-2 and Bax mRNAs were determined by RT-PCR. Protein expressions of HIF-1α, VEGF, and VEGFR were determined by Western blot. Results D0, Dq and SF2 value of KYSE-150 cells decreased along with the concentration of rhES. At D0 dose, the SER for 100 and 200 μg/ml rhES was 1.14 and 1.27, respectively. Compared with the radiation alone group, the apoptosis rate and bax expression increased, while the expressions of VEGF and HIF-1α decreased in the combination group (t=7.97, 3.02, 117.55, 7.22, P<0.05). Conclusions rhES has radiosensitive effect on esophageal carcinoma cells KYSE-150 in vitro by inhibiting the expressions of HIF-1α and VEGF, regulating bax expression, and inducing apoptosis. |
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