中华放射医学与防护杂志

陈小宇,祝爱珍,刘成成,朱锦灿,刘革修.1,25-(OH)₂D₃对辐射损伤小鼠骨髓微环境的保护作用[J].中华放射医学与防护杂志,2013,33(4):337-340

1,25-(OH)₂D₃对辐射损伤小鼠骨髓微环境的保护作用

Protective effect of 1,25-(OH)₂D₃on radiation-induced bone marrow microenvironment injury

投稿时间：2013-01-29

DOI：10.3760/cma.j.issn.0254-5098.2013.04.001

英文关键词:Bone marrow microenvironment Radiation Adipogenesis 1,25-(OH)₂D₃

基金项目:国家自然科学基金(81270568)

作者	单位	E-mail
陈小宇	510632 广州, 暨南大学血液病研究所
祝爱珍	510632 广州, 暨南大学血液病研究所
刘成成	510632 广州, 暨南大学血液病研究所
朱锦灿	510632 广州, 暨南大学血液病研究所
刘革修	510632 广州, 暨南大学血液病研究所	tliugx@jnu.edu.cn

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中文摘要:

目的探讨1,25-(OH)₂D₃对辐射损伤小鼠骨髓微环境的保护作用,并研究其可能的分子机制。方法 60只雄性7周龄健康BALB/c小鼠按随机区组法分为健康对照组、单纯照射组和照射+给药组,每组20只。单纯照射组和照射+给药组给予6.0 Gy ⁶⁰Co γ射线照射,照射+给药组于照射前48 h开始给予1,25-(OH)₂D₃ 2.5 μg/kg(用DMSO稀释)灌胃至辐射后第8天,单纯照射组给予相同剂量DMSO对照。干预后观察小鼠的一般情况、体重变化、外周血象,并取股骨制作骨髓切片观察骨髓病理改变,测定脂肪细胞面积,免疫组织化学染色比较照射后第8天不同组间过氧化物酶体增殖活化受体γ(PPARγ)的表达。结果接受照射后小鼠均出现体重减轻,外周血白细胞降低,骨髓脂肪化等骨髓造血抑制改变。与单纯照射组比较,1,25-(OH)₂D₃能改善小鼠一般情况,减轻体重下降(t_{照射后3 d、6 d}=-2.23、-2.34,P<0.05);另外,照射后第4天和第8天,照射+给药组小鼠外周血白细胞计数高于单纯照射组(t=-4.99、-4.46,P<0.05);脂肪空泡面积减少(t=-3.75、-2.10,P<0.05);采用免疫组织化学染色发现1,25-(OH)₂D₃通过抑制PPARγ表达,减少骨髓脂肪化的形成。结论 1,25-(OH)₂D₃可抑制辐射后骨髓脂肪化,保护辐射后小鼠骨髓微环境。

英文摘要:

Objective To investigate the protective effect of 1,25-(OH)₂D₃ on radiation-induced bone marrow microenvironment injury and to explore the related molecular mechanism. Methods Sixty 7-week old male BALB/c mice were randomly divided into control group without any treatment; radiation group exposed to 6.0 Gy ⁶⁰Co γ-rays with DMSO, and 1,25-(OH)₂D₃+radiation group treated with 1,25-(OH)₂D₃2.5 μg/kg dissolved in DMSO each day and 6 Gy of γ-rays. The body weight and peripheral white blood cells, femur bone marrow histology, and the proportion of adipocyte area were measured. The expression of peroxisome proliferator-activated receptor-gamma (PPARγ) was detected immunohistochemistrically at 8 d after irradiation. Results After irradiation, the number of white blood cells and the body weight decreased obviously, and the percentage of adipocyte area was increased significantly. Compared with radiation group, 1,25-(OH)₂D₃ reduced the decrease rate of body weight (t=-2.23,-2.34,P<0.05), partly recovered the number of white blood cells at 4 or 8 d after irradiation(t=-4.99,-4.46,P<0.05), and reduced the proportion of adipocyte area (t=-3.75,-2.10, P<0.05). With immunohistochemistrical assay, it was found that 1,25-(OH)₂D₃ inhibited adipogenesis by reducing the expression of PPARγ. Conclusions 1,25-(OH)₂D₃ decreases radiation-induced adipogenesis and hence protects the bone marrow microenvironment from radiation damage.

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