中华放射医学与防护杂志

张丽丽,刘希光,赵如森,等.凋亡素2配体对食管癌细胞株Eca-109放射敏感性的影响[J].中华放射医学与防护杂志,2013,33(3):278-281.ZHANG Li-li,LIU Xi-guang,ZHAO Ru-sen,et al.Radiosensitive effect of TRAIL on esophageal cancer cell line-Eca109[J].Chin J Radiol Med Prot,2013,33(3):278-281

凋亡素2配体对食管癌细胞株Eca-109放射敏感性的影响

Radiosensitive effect of TRAIL on esophageal cancer cell line-Eca109

投稿时间：2012-10-19

DOI：10.3760/cma.j.issn.0254-5098.2013.03.015

中文关键词: 凋亡素2配体食管癌细胞放射敏感性凋亡 G₂/M期阻滞

英文关键词:TRAIL Esophageal cancer cell Radiosensitivity Apoptosis G₂/M Arrest

基金项目:

作者	单位	E-mail
张丽丽	266003 青岛大学医学院附属医院肿瘤科
刘希光	266003 青岛大学医学院附属医院肿瘤科	liuxiguang1962@yahoo.com.cn
赵如森	临淄区人民医院肿瘤科
宋浩	266003 青岛大学医学院附属医院肿瘤科
张红军	266003 青岛大学医学院附属医院肿瘤科
王玉坤	266003 青岛大学医学院附属医院肿瘤科
李敬东	临沂市人民医院肿瘤科
李伟	266003 青岛大学医学院附属医院肿瘤放射治疗部
梁晔	266003 青岛大学医学院附属医院中心实验室
王相	266003 青岛大学医学院附属医院肿瘤放射治疗部

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中文摘要:

目的研究凋亡素2配体(Apo-2 ligand,Apo2L),又称肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-related apoptosis inducing ligand,TRAIL)对体外培养食管癌细胞株Eca-109放射敏感性的影响。方法 MTT法检测TRAIL对Eca-109细胞的毒性;克隆形成实验检测TRAIL对Eca-109细胞的放射增敏作用;流式细胞仪(FCM)技术分析TRAIL对凋亡率及细胞周期的影响。结果 100~800 μg/ml TRAIL随浓度升高对Eca-109细胞的增殖抑制率增大,药物浓度与细胞抑制率正相关(r=0.981,P<0.01),50%抑制浓度(IC₅₀)为637 μg/ml;200 μg/ml TRAIL能增加Eca-109细胞的放射敏感性,放射增敏比为1.219;不同剂量(0~8 Gy)照射后,给药组的凋亡率均高于单照射组(F=16.97、76.65、92.86、209.66,P<0.05),给药组G₂/M期细胞比例较单照射组增加(F=9.40、84.99、87.61、2025.85,P<0.05)。结论 TRAIL可以抑制Eca-109细胞的生长,诱导细胞凋亡和G₂/M期阻滞,后者可能与TRAIL的放射增敏机制有关。

英文摘要:

Objective To study the radiosensitive effect of Apo-2 ligand (Apo2L),known as tumor necrosis factor-related apoptosis inducing ligand (TRAIL), on esophageal cancer cell line-Eca109 in vitro.Methods MTT assay and clonogenic assay were performed to evaluate the cytotoxicity and radiosensitization of TRAIL, respectively. Cell apoptosis and cell cycle distribution were measured by flow cytometry (FCM). Results TRAIL inhibited cell growth in a dose-dependent manner and its 50% inhibition concentration(IC₅₀) was 637 μg/ml. The concentration 200 μg/ml TRAIL could enhance cell radiosensitivity with a SER of 1.219. After 2, 4, 6, 8 Gy X-ray irradiation, the incidence of apoptosis in TRAIL-treated cells was higher than that of irradiation alone groups(F=16.97, 76.65, 92.86, 209.66,P<0.05). The percentage of cells in G₂/M phase of TRAIL-treated groups was also higher than that of irradiation alone groups(F=9.40, 84.99, 87.61, 2025.85,P<0.05). Conclusions TRAIL could inhibit cell growth, induce apoptosis and G₂/M arrest, and had radiosensitization effect on Eca-109 cells.

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