| 甘曹辉,朱国英,陈晓,等.137Cs γ射线诱导人外周血线粒体DNA缺失的时间和剂量-效应关系[J].中华放射医学与防护杂志,2013,33(3):273-277.GAN Cao-hui,ZHU Guo-ying,CHEN Xiao,et al.Time-and dose-effect of mitochondrial DNA deletions in γ-ray irradiated human peripheral blood[J].Chin J Radiol Med Prot,2013,33(3):273-277 |
| 137Cs γ射线诱导人外周血线粒体DNA缺失的时间和剂量-效应关系 |
| Time-and dose-effect of mitochondrial DNA deletions in γ-ray irradiated human peripheral blood |
| 投稿时间：2012-11-12 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.014 |
| 中文关键词: 电离辐射 线粒体DNA 4934 bp缺失 4977 bp缺失 时间-效应 剂量-效应 |
| 英文关键词:Ionizing radiation Mitochondrial DNA deletions 4934bp deletion Time-effect Dose-effect |
| 基金项目:上海市公共卫生重点学科建设项目(12GWZX0401) |
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| 中文摘要: |
| 目的 研究137Cs γ射线诱导的人外周血线粒体DNA 4934 bp和4977 bp缺失的时间和剂量-效应,并探讨其在电离辐射受照者剂量估算中的应用意义。方法 采集5名健康成人外周血进行137Cs γ射线离体照射,取其中1份血样给予5 Gy照射后分别培养2、24、48和72 h,另4份血样均经6等分后分别给予0、0.5、1、2、5和10 Gy照射,孵养2 h后采用实时荧光定量PCR方法和凝胶电泳,检测人外周血线粒体DNA 4934 bp(mtDNA 4934 bp)和4977 bp(mtDNA 4977 bp)缺失的表达水平,并用Curve Expert 1.4软件拟合剂量-效应曲线。结果 137Cs γ射线照射离体人血后,诱发的mtDNA 4934 bp 缺失和mtDNA 4977 bp 缺失在照后2 h即升高,mtDNA 4934 bp缺失水平在照后2 h和48 h有相对表达高点(t=10.782和8.966, P<0.05);而mtDNA 4977 bp缺失水平在照后48 h表达最高(t=7.433, P<0.05)。0.5~10 Gy的137Cs γ射线照射诱发的mtDNA 4934 bp 缺失(t=2.895~8.105, P<0.05)和mtDNA 4977 bp 缺失(t=3.006~7.715, P<0.05)均随照射剂量增加。其中,mtDNA 4977 bp缺失在相同照射剂量下变化更大,尤其是在10 Gy剂量照射时,二者差异更明显(t=2.919, P<0.05),即对于大剂量照射,mtDNA 4977 bp缺失可能更为灵敏,但是个体差异较mtDNA 4934 bp缺失大。拟合的剂量-效应曲线回归方程分别为Ŷ1=1.178+0.1219D(R2=0.9269, mtDNA 4934 bp缺失)和Ŷ2=1.2578+0.1933D(R2=0.9016, mtDNA 4977 bp缺失)。结论 137Cs γ射线诱发的线粒体DNA片段缺失与辐射剂量有良好的数学回归关系,有可能为照射后生物剂量快速估算和预后评估提供依据。 |
| 英文摘要: |
| Objective To study the time-and dose-effect of mitochondrial DNA (mtDNA) 4934 bp and 4977 bp deletions in the human peripheral blood irradiated by137Cs γ-rays, and to evaluate its implication in biological dosimetry.Methods The peripheral blood from five healthy adults was collected and irradiated with γ-rays. The peripheral blood of one healthy adult was irradiated with 5 Gy and cultured for 2, 24, 48 and 72 h after irradiation. The peripheral blood from the other four healthy adults was cultured for 2 h after 0, 0.5, 1, 2, 5 and 10 Gy irradiation. The peripheral blood mtDNA 4934 bp and 4977 bp deletions were detected by real-time polymerase chain reaction and gel electrophoresis. The dose-effect curves were fitted using Curve Expert 1.4 Software. Results mtDNA 4934 bp and 4977 bp deletions were induced at 2 h post-irradiation and the mtDNA 4934 bp deletion had relative high levels at 2 h and 48 h after radiation (t=10.782 and 8.966, P<0.05), and mtDNA 4977 bp deletion reached the highest level at 48 h after radiation (t=7.433, P<0.05). mtDNA 4934 bp (t=2.895-8.105, P<0.05) and 4977 bp deletion (t=3.006-7.715, P<0.05) irradiated at 0.5-10 Gy increased with a dose-dependent manner. The incidence of mtDNA 4977 bp deletion was higher than that of 4934 bp deletion for those samples exposed with same dose of irradiation, especially at 10 Gy (t=2.919, P<0.05), which suggested that 4977 bp deletion might be more sensitive than 4934 bp deletion at high dose. But larger individual differences were found in 4977 bp deletion compared with 4934 bp deletion. The dose-effect equations for 4934 bp deletion and 4977 bp deletion were Ŷ1=1.178+0.1219D (R2=0.9269) and Ŷ2=1.2578+0.1933D (R2=0.9016), respectively.Conclusions The induction of mtDNA deletion was correlated with radiation dose, and thus it may be a available method for biological dose estimation and prognostic evaluation. |
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