| 范莉,陈德清,刘青杰,等.硫辛酸氨基酸盐对L02和AHH-1细胞辐射防护作用的研究[J].中华放射医学与防护杂志,2013,33(3):269-272.FAN Li,CHEN De-qing,LIU Qing-jie,et al.Radioprotection effect of lipoic acid amino acid salts on L20 and AHH-1 cells[J].Chin J Radiol Med Prot,2013,33(3):269-272 |
| 硫辛酸氨基酸盐对L02和AHH-1细胞辐射防护作用的研究 |
| Radioprotection effect of lipoic acid amino acid salts on L20 and AHH-1 cells |
| 投稿时间：2013-01-25 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.013 |
| 中文关键词: 硫辛酸氨基酸盐 硫辛酸精氨酸盐 电子自旋共振光谱 CCK-8 抗辐射 |
| 英文关键词:Lipoic acid amino acid salt Lipoic acid arginine salt ESR CCK-8 Radioprotection |
| 基金项目:卫生行业科研专项(201002009);中国疾病预防控制中心青年科研基金(2009A203) |
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| 中文摘要: |
| 目的 通过对不同结构硫辛酸氨基酸盐进行抗辐射效应研究,为开发新型安全有效的抗辐射药提供实验依据。方法 通过芬顿体系利用电子自旋共振光谱(ESR)检测硫辛酸氨基酸盐清除自由基的能力;采用Cell Counting Kit-8(CCK-8)法检测其对γ射线照射后人肝L02细胞存活率的影响;流式细胞分析法研究其对γ射线照射后人淋巴细胞AHH-1凋亡变化的影响。结果 ESR清除自由基实验显示硫辛酸精氨酸盐清除自由基能力强于硫辛酸及其他硫辛酸氨基酸盐,IC50值为8.40 μmol/ml。CCK-8法实验结果显示硫辛酸氨基酸盐与硫辛酸的抗辐射能力相当,且稳定性更好(P>0.05)。流式细胞分析法对AHH-1进行细胞凋亡检测,硫辛酸氨基酸盐均表现抑制凋亡作用,硫辛酸精氨酸盐作用最好(t=-6.67,P<0.01)。结论 硫辛酸精氨酸盐对L02和AHH-1细胞有较好的抗辐射作用, 其作用机制可能与其清除自由基的作用有关。 |
| 英文摘要: |
| Objective For developing safe and effective anti-radiation new drugs, the effects of different lipoic acid amino acid salts on radiosensitivity were investigated.Methods The free radical scavenging ability of the above salts was evaluated in Fenton system. CCK-8 assay and flow cytometry assay were performed to evaluate the survival rate of L02 and apoptosis of AHH-1 after γ-ray irradiation, respectively. Results The lipoic acid arginine salt had the best ability of scavenging free radicals in Fenton system with an IC50 of 8.40 μmol/ml. The survival assay showed that lipoic acid amino acid salts had better stability and equal ability in radioprotection (P>0.05) compared with lipoic acid. The apoptosis assay indicated that all lipoic acid amino acid salts could inhibit radiation-induced apoptosis, where lipoic acid arginine salt was more effective (t=-6.67, P<0.01). Conclusions Lipoic acid arginine salt has good radioprotection effect on L02 and AHH-1 cells by scavenging free radicals. |
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