中华放射医学与防护杂志

倪昕晔,钱农,林涛,等.甘氨双唑钠修饰纳米金对肺腺癌细胞A549的放射增敏研究[J].中华放射医学与防护杂志,2013,33(3):265-268.NI Xin-ye,QIAN Nong,LIN Tao,et al.Radiosensitization effect of gold nanoparticles modified by sodium glycididazole on lung adenocarcinoma cell A549[J].Chin J Radiol Med Prot,2013,33(3):265-268

甘氨双唑钠修饰纳米金对肺腺癌细胞A549的放射增敏研究

Radiosensitization effect of gold nanoparticles modified by sodium glycididazole on lung adenocarcinoma cell A549

投稿时间：2012-08-09

DOI：10.3760/cma.j.issn.0254-5098.2013.03.012

中文关键词: 甘氨双唑钠纳米金放射增敏肺腺癌细胞/A549

英文关键词:Sodium glycididazole Gold nanoparticles Radiosensitization Lung adenocarcinoma cell/A549

基金项目:

作者	单位
倪昕晔	213003, 南京医科大学附属常州第二人民医院放疗科
钱农	213003, 南京医科大学附属常州第二人民医院放疗科
林涛	213003, 南京医科大学附属常州第二人民医院放疗科
聂斌	213003, 南京医科大学附属常州第二人民医院放疗科
孙志强	213003, 南京医科大学附属常州第二人民医院放疗科
孙苏平	213003, 南京医科大学附属常州第二人民医院放疗科

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中文摘要:

目的探讨甘氨双唑钠修饰纳米金制备及放射增敏效果。方法把甘氨双唑钠修饰到已连接聚乙二醇的纳米金上,纳米金粒径18 nm。利用扫描电镜观察肺腺癌(A549)细胞吞噬甘氨双唑钠-纳米金的现象。将培养的肺腺癌细胞分为甘氨双唑钠组、纳米金组、甘氨双唑钠-纳米金组、对照组(不加药组)。用四甲基偶氮唑盐比色法和克隆形成实验对肺腺癌(A549)细胞进行放射增敏的研究。结果甘氨双唑钠-纳米金能进入细胞质和细胞核;浓度为0.003 mg/ml纳米金和甘氨双唑钠-纳米金没有明显的细胞毒性;甘氨双唑钠-纳米金组相对于甘氨双唑钠组、纳米金组、对照组,D₀、D_q出现了下调;接受2、4、6和8 Gy剂量照射后,甘氨双唑钠-纳米金组肺腺癌细胞的存活率与其他3组相比,均明显下降(F=4.8、14.5、5.7、7.6,P<0.05)。结论甘氨双唑钠修饰的纳米金能增加肺腺癌细胞的辐射敏感性。

英文摘要:

Objective To study the radiosensitization effect of gold nanoparticles modified by sodium glycididazole. Methods The sodium glycididazole was connected to gold nanoparticle, in dimension of about 18 nm, that had been modified with polyethylene glycol. The nanoparticle-swallowing efficiency of lung adenocarcinoma A549 cells was observed by a scanning electron microscope. Cells were divided into four groups: sodium glycididazole group, gold nanoparticles group, sodium glycididazole-gold nanoparticles group, and no drug control group. The radiosensitivity was detected by MTT and colony formation assays. Results Sodium glycididazole-gold nanoparticles could enter the cell cytoplasm and nucleus. The concentration of 0.003 mg/ml gold nanoparticles and sodium glycididazole-gold nanoparticles had no obvious cytotoxic effect. After irradiation of 2, 4, 6, 8 Gy, the cell survival of the sodium glycididazole-gold nanoparticle group was significantly lower than that of the other three groups (F=4.8, 14.5, 5.7, 7.6,P<0.05) and the D₀ and D_q values of the sodium glycididazole-gold nanoparticle group were significantly lower than those of other three groups. Conclusion Sodium glycididazole-gold nanoparticles can enhance the radiosensitivity of lung adenocarcinoma cells.

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