中华放射医学与防护杂志

高刚,杨英杰,刘建香,等.α粒子诱发人支气管上皮细胞恶性转化不同时期差异表达microRNAs的筛选[J].中华放射医学与防护杂志,2013,33(3):261-264.GAO Gang,YANG Ying-jie,LIU Jian-xiang,et al.Differentially expressed microRNAs in the early and late transformed cells and their parental BEP2D cells[J].Chin J Radiol Med Prot,2013,33(3):261-264

α粒子诱发人支气管上皮细胞恶性转化不同时期差异表达microRNAs的筛选

Differentially expressed microRNAs in the early and late transformed cells and their parental BEP2D cells

投稿时间：2012-09-24

DOI：10.3760/cma.j.issn.0254-5098.2013.03.011

中文关键词: α粒子人支气管上皮细胞微小RNA

英文关键词:α-particles Human bronchial epithelial cells microRNAs

基金项目:卫生行业科研专项(201002009)

作者	单位	E-mail
高刚	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室
杨英杰	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室
刘建香	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室
田梅	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室
苏旭	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室	suxu@nirp.cn

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中文摘要:

目的筛选α粒子诱发人支气管上皮细胞恶性转化不同时期差异表达的microRNAs。方法用microRNAs芯片筛选α粒子诱发人支气管上皮细胞恶性转化不同时期差异表达的microRNAs。结果与 BEP2D细胞相比,R15H20细胞中存在38个差异表达microRNAs,其中18个表达上调,20个表达下调;与 BEP2D细胞相比,RHT35细胞中存在87个差异表达microRNAs,其中47个表达上调,40个表达下调;与 R15H20细胞相比,RHT35细胞中存在38个差异表达microRNAs,其中20个表达上调,18个表达下调;与 BEP2D细胞相比,R15H20和RHT35细胞中变化一致的microRNAs有25个,其中10个表达上调,15个表达下调。结论 microRNAs表达异常与α粒子诱发人支气管上皮细胞恶性转化密切相关。

英文摘要:

Objective To identify the differentially expressed microRNAs in the early transformed cells, the late transformed cells and their parental BEP2D cells.Methods The differentially expressed microRNAs in the above cells were identified by microRNAs microarray assay. Results There were 38 differentially expressed microRNAs in R15H20 cells versus BEP2D cells, with 18 upregulated and 20 downregulated microRNAs. R15H20 and RHT35 cells shared 25 differentially expressed microRNAs compared with BEP2D cells, with 15 down-regulated and 10 up-regulated microRNAs. There were 87 differentially expressed microRNAs in RHT35 cells versus BEP2D cells, with 47 upregulated and 40 downregulated microRNAs. There were 38 differentially expressed microRNAs in RHT35 cells versus R15H20 cells with 20 upregulated and 18 downregulated microRNAs.Conclusions microRNAs are differentially expressed in the different stages of carcinogenesis of BEP2D cells induced by α particles, which suggests that microRNAs may play an important role in α particle-induced malignant transformation of BEP2D cells.

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