| 叶爽,袁德晓,谢月霞,等.长期低剂量γ照射对人B淋巴母细胞辐射敏感性的影响[J].中华放射医学与防护杂志,2013,33(3):256-260.YE Shuang,YUAN De-xiao,XIE Yue-xia,et al.Effects of long-term low-dose γ-rays exposure on radiosensitivity of human B lymphoblast cells[J].Chin J Radiol Med Prot,2013,33(3):256-260 |
| 长期低剂量γ照射对人B淋巴母细胞辐射敏感性的影响 |
| Effects of long-term low-dose γ-rays exposure on radiosensitivity of human B lymphoblast cells |
| 投稿时间：2013-01-10 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.010 |
| 中文关键词: 低剂量辐射 细胞增殖 辐射敏感性 凋亡 基因表达 |
| 英文关键词:Low-dose-radiation Cell proliferation Radiosensitivity Apoptosis Gene expressions |
| 基金项目:国家自然科学基金(81273001,11179002,31070758) |
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| 中文摘要: |
| 目的 探讨长期低剂量(LDR)γ辐射对人B淋巴母细胞HMy2.CIR(HMy)辐射敏感性的影响及其机制。方法 实验将HMy细胞分为对照组和长期LDR组,其中长期LDR组选用可以显著促进细胞增殖的低剂量γ射线对HMy细胞每周照射3次,每次0.032 Gy,连续照射4周,在长期LDR处理结束后分别对部分对照组和长期LDR组细胞进行2 Gy照射。以CCK-8[内含WST-8,即2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺酸苯)-2H-四唑单钠盐]法检测细胞增殖和辐射敏感性,流式细胞术检测细胞凋亡率和γ-H2AX表达,RT-PCR法检测细胞周期相关基因cyclinD1、细胞增殖核抗原PCNA,以及凋亡相关基因bcl-2和bax的表达。结果 长期LDR可以显著提高细胞的增殖率(t=9.607,P<0.01), 增加cyclinD1和PCNA基因表达(t=6.869、9.229,P<0.01),增加抗凋亡基因bcl-2表达(t=2.662,P<0.05),降低抑凋亡基因bax的表达(t=19.908,P<0.01)。同时,受长期LDR照射的细胞对攻击剂量(2 Gy)产生适应性,使得细胞辐射敏感性显著降低(t=8.896,P<0.01);与单纯2 Gy照射组相比,LDR+2 Gy组细胞γ-H2AX表达量(t=10.264,P<0.01)和细胞凋亡率(t=4.762,P<0.01)均显著降低。结论 长期LDR辐射可通过促进周期相关基因表达从而促进细胞增殖,并通过减少细胞凋亡来降低其辐射敏感性。 |
| 英文摘要: |
| Objective To investigate the effects of long-term low-dose radiation (LDR) of γ-rays on the proliferation and radiosensitivity of human lymphoblast cells HMy2.CIR (HMy) and to elucidate the underlying mechanism.Methods HMy cells were divided into control group and long-term LDR group. For the long-term LDR treatment, HMy cells were fractionally exposed to a low dose of γ-rays, which could enhance cell proliferation, 3 times per week for 4 weeks. After the long-term LDR exposure, part of the control and long-term LDR exposed cells were further irradiated with a challenging dose (2 Gy) of γ-rays. Then cell proliferation and radiosensitivity were assayed by CCK-8 kit, cell apoptosis, and γ-H2AX formation was measured by flow cytometry. Gene expressions of cyclinD1, PCNA, bcl-2 and bax were detected by RT-PCR. Results The long-term LDR significantly increased cell proliferation (t=9.607,P<0.01) accompanied with up-regulation of cell cycle regulation gene cyclinD1 (t=6.869, P<0.01), proliferation regulation gene PCNA (proliferating cell nuclear antigen) (t=9.229, P<0.01) and bcl-2 gene (t=2.662,P<0.05), but decreased the expression of pro-apoptotic gene bax (t=19.908, P<0.01) in HMy cells. Compared to untreated cells, the long-term LDR decreased cell radiosensitivity (t=8.896,P<0.01), including apoptosis induction (t=4.762,P<0.01) and γ-H2AX formation (t=10.264, P<0.01). Conclusions The long-term LDR promoted cell proliferation by up-regulating cell cycle related genes, while it reduced the radiosensitivity of HMy cells with acquisition of apoptotic resistance. |
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