| 付岳,杜利清,王彦,等.白藜芦醇抑制辐射诱导的IL-1β表达[J].中华放射医学与防护杂志,2013,33(3):239-241.FU Yue,DU Li-qing,WANG Yan,et al.Effect of resveratrol on the expression of IL-1β in mesenchymal stem cells exposed to ionizing radiation[J].Chin J Radiol Med Prot,2013,33(3):239-241 |
| 白藜芦醇抑制辐射诱导的IL-1β表达 |
| Effect of resveratrol on the expression of IL-1β in mesenchymal stem cells exposed to ionizing radiation |
| 投稿时间：2013-02-04 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.005 |
| 中文关键词: 白藜芦醇 间充质干细胞 电离辐射 白介素-1β |
| 英文关键词:Resveratrol Mesenchymal stem cells Ionizing radiation IL-1β |
| 基金项目:国家自然科学基金(31200634, 31170804, 31240052);卫生行业科研专项(201002009);天津市自然科学基金(11ZCGYSY02400, 12JCYBJC15300, 12JCYBJC32900) |
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| 中文摘要: |
| 目的 研究白藜芦醇对间充质干细胞(MSCs)受照后产生的炎症因子IL-1β的影响,探讨白藜芦醇对MSCs的辐射防护作用。方法 将间充质干细胞分为空白对照组、单纯照射组与白藜芦醇组,白藜芦醇组预先给予不同浓度的白藜芦醇,经137Csγ射线照射后,应用ELISA、Western blot和 RT-PCR等方法检测 IL-1β的分泌和表达。结果 预先给予间充质干细胞50、100和200 μmol/L的白藜芦醇组与单纯照射组相比,IL-1β胞外分泌显著降低(t=83.34、24.48、12.52,P<0.05),同时细胞内IL-1β蛋白表达和mRNA水平也显著降低(t=8.69、14.77、13.90,P<0.05)。结论 白藜芦醇可明显抑制辐射诱导的间充质干细胞炎症因子IL-1β的产生。 |
| 英文摘要: |
| Objective To investigate the effect of resveratrol on IL-1β production in mesenchymal stem cells (MSCs) exposed to radiation and evaluate the radioprotective effect of resveratrol. Methods MSCs were divided into non-treated control group, irradiated group and resveratrol-treated group where resveratrol with different dosage of 50, 100 and 200 μmol/L was administrated to MSCs before irradiation. The expressions of IL-1β mRNA and protein were detected using real time PCR, ELISA, and Western blot, respectively. Results Compared to the irradiation group, pretreatment of cell with resveratrol reduced the external secretion of IL-1β (t=83.34, 24.48, 12.52, P<0.05) as well as the intracellular expression of IL-1β protein and mRNA (t=8.70,14.77,13.90, P<0.05).Conclusions Resveratrol could inhibit the induction of inflammatory factor IL-1β in the irradiated MSCs. |
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