中华放射医学与防护杂志

吴广银,侯盘长,王伟,等.人食管癌EC9706细胞乏氧环境中放射敏感性变化及其机制[J].中华放射医学与防护杂志,2013,33(2):138-141.WU Guang-yin,HOU Pan-chang,WANG Wei,et al.Radioresistance change and the mechanism of human esophaged cancer EC9706 cells in hypoxia[J].Chin J Radiol Med Prot,2013,33(2):138-141

人食管癌EC9706细胞乏氧环境中放射敏感性变化及其机制

Radioresistance change and the mechanism of human esophaged cancer EC9706 cells in hypoxia

投稿时间：2012-11-01

DOI：10.3760/cma.j.issn.0254-5098.2013.02.007

中文关键词: 食管癌乏氧诱导因子-α 血管内皮生长因子-A 血管内皮生长因子-D

英文关键词:Esophageal cancer Hypoxia-inducible factor-1 alpha (HIF-1α) Vascular endothelial growth factor-A (VEGF-A) Vascular endothelial growth factor-D (VEGF-D)

基金项目:河南省科技厅科技基础与前沿技术研究计划项目(112300410296)

作者	单位	E-mail
吴广银	450003 郑州,河南省人民医院放疗科
侯盘长	450003 郑州,河南省人民医院放疗科
王伟	450003 郑州,河南省人民医院放疗科
雒建超	450003 郑州,河南省人民医院放疗科
朱绍成	450003 郑州,河南省人民医院放疗科	zsc2686@yahoo.com.cn

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中文摘要:

目的观察乏氧干预对人食管癌细胞EC9706放射敏感性及乏氧诱导因子-1α(HIF-1α)、血管内皮生长因子-A (VEGF-A)和血管内皮生长因子-D (VEGF-D)表达的影响,以及乏氧诱导因子-1α和血管内皮生长因子表达的相关性。方法将人食管癌细胞EC9706体外常氧及乏氧培养6、12、24 h,置于6 MV X射线下照射0、1、2、4、6、8 Gy,细胞克隆法绘制生长曲线;Western blot法检测常氧及乏氧培养6、12、24 h后各组HIF-1α、VEGF-A、VEGF-D蛋白表达情况;RNA干扰沉默HIF-1α后乏氧培养6、12、24 h,再以Western blot法检测各组HIF-1α、VEGF-A、VEGF蛋白表达情况。结果乏氧组细胞存活分数(SF₂)为0.62,常氧组SF₂为0.43,而且乏氧组HIF-1α、VEGF-A、VEGF-D蛋白表达量随乏氧时间的延长逐渐增加(F=205.24、227.88、130.55,P<0.05)。RNA干扰后,乏氧EC9706细胞中HIF-1α、VEGF-A、VEGF-D蛋白表达量均未见升高。结论乏氧环境下食管癌细胞EC9706对放射敏感性下降,这可能与乏氧诱导的HIF-1α及其下游因子VEGF-A和VEGF-D的蛋白表达增加相关。

英文摘要:

Objective To investigate the expression of hypoxia-inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor-A (VEGF-A) and vascular endothelial growth factor-D (VEGF-D) in hypoxic environment as well as the relationship between HIF-1α and VEGF-D. Methods Human esophageal cancer cell line EC9706 was cultured under hypoxia environment for 6, 12 and 24 h, the cell radiosensitivity was evaluated by survival curve. HIF-1α siRNA was constructed and transfected into human EC9706 cells. Protein expressions of HIF-1α, VEGF-A and VEGF-D were analyzed by Western blot before and after RNA interference. Results EC9706 cells under hypoxia showed radioresistance with a SF₂ of 0.62 higher than that of normoxic cells of 0.43. Moreover, the protein expressions of HIF-1α, VEGF-A and VEGF-D were all increased (F=205.24, 227.88, 130.55,P<0.05) due to hypoxia treatment. On the contrary, after HIF-1α siRNA transfer, the protein expressions of HIF-1α, VEGF-A and VEGF-D in EC9706 cells were not influenced by hypoxia treatment.Conclusions EC9706 cells in hypoxic environment was radioresistance, and the upexpressions of HIF1α, VEGF-A and VEGF-D may be involved.

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