| 江茂情,陈皓鋆,豆晓峰,等.18F-FLT和18F-FDG评价食管癌细胞照射后超早期生物学反应[J].中华放射医学与防护杂志,2013,33(2):131-134.JIANG Mao-qing,CHEN Hao-jun,DOU Xiao-feng,et al.Using 18F-FLT and 18F-FDG to evaluate the ultra-early biological response of esophageal carcinoma cells after irradiation[J].Chin J Radiol Med Prot,2013,33(2):131-134 |
| 18F-FLT和18F-FDG评价食管癌细胞照射后超早期生物学反应 |
| Using 18F-FLT and 18F-FDG to evaluate the ultra-early biological response of esophageal carcinoma cells after irradiation |
| 投稿时间：2012-10-24 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.02.005 |
| 中文关键词: 18F-氟代脱氧葡萄糖 18F-氟代脱氧胸腺嘧啶 食管癌细胞 生物学反应 |
| 英文关键词:18F-FDG 18F-FLT Esophageal carcinoma cells Biological response |
| 基金项目:福建省自然科学基金(2008-59-11);厦门市科技计划指导性项目(3502720077056) |
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| 中文摘要: |
| 目的 比较18F-氟代脱氧胸腺嘧啶(18F-FLT)和18F-氟代脱氧葡萄糖(18F-FDG)在反映食管癌细胞受照后超早期生物学反应的差异。方法 将人食管癌Eca-109细胞分别接受5、10、15 Gy剂量X射线照射,照射后2、4、8 h检测细胞对18F-FDG 和18F-FLT摄取率的变化,以及细胞相对存活率和ATP表达情况的变化。结果 5 Gy照射后2、4 h,细胞对18F-FDG摄取率与对照组相比分别减少了9.45%和16.4%,差异无统计学意义;但15 Gy照后2 h,对18F-FDG摄取率却增加了26.5%(t=3.04,P＜0.05),其余照射组对18F-FDG摄取率均有明显减少(F=25.75,P＜0.05)。5 Gy照后2 h,18F-FLT摄取率(3.65±0.41)%与对照组(4.00±0.42)%相比,差异无统计学意义,其余照射组对18F-FLT摄取率与对照组比较均有明显减少(F=33.93,P＜0.05)。在5、10、15 Gy照后2、4、8 h,各组细胞相对存活率差异无统计学意义。经不同剂量照射后,细胞对18F-FLT摄取率与ATP浓度之间的相关性(r=0.887,P＜0.05)优于18F-FDG与ATP浓度之间的相关性(r=0.622,P>0.05)。 结论 18F-FDG 和18F-FLT两者均可反映食管癌细胞照射后超早期生物学反应,18F-FLT比18F-FDG 能较好地反映食管癌细胞照射后超早期生物学反应。 |
| 英文摘要: |
| Objective To compare the difference of 3'-deoxy-3'-18F-fluorothymidine (18F-FLT) and 2'-deoxy-2'-18F-fluoro-D-glucose (18F-FDG) in the ultra-early biological response of esophageal carcinoma cells after irradiation. Methods After 2, 4 and 8 h of irradiation with different doses (5, 10, 15 Gy), the uptake ratio of 18F-FDG and 18F-FLT, survival, and ATP level of esophageal carcinoma cells were detected. Results After 2 and 4 h of 5 Gy irradiation, the cellular 18F-FDG uptake ratio was decreased by 9.45% and 16.4%. After 2 h of 15 Gy irradiation, this uptake ratio was increased by 26.5% (t=3.04, P<0.05). But the 18F-FDG uptake ratios of the other experimental groups were decreased remarkably (F=25.75, P<0.05). On the other side, after 2 h of 5 Gy irradiation, the 18F-FLT uptake ratio had no statistically difference [(3.65±0.17)% vs (4.00±0.17)%, P>0.05], and 18F-FLT uptake ratios of the other groups were decreased notably (F=33.93, P<0.05). The relative survival of esophageal carcinoma cells after 2, 4 and 8 h of irradiation with different doses (5, 10, 15 Gy) has no significant difference. Line regression analysis indicated a significant correlation between 18F-FLT and ATP levels (r=0.887, P<0.05), whereas a less correlation was found between 18F-FDG and ATP levels (r=0.622, P>0.05). Conclusions Both 18F-FDG and 18F-FLT can evaluate the ultra-early irradiation response of esophageal carcinoma cells, but 18F-FLT may be more specific than 18F-FDG in monitoring the ultra-early irradiation response. |
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