中华放射医学与防护杂志

朱雅群,廖岩森,章斌,等.¹⁸F-FLT细胞摄取率评价结直肠癌HCT116细胞早期放射反应[J].中华放射医学与防护杂志,2013,33(1):27-31.ZHU Ya-qun,LIAO Yan-sen,ZHANG Bin,et al.Investigation of early radiation response by evaluating the capability of ¹⁸F-FLT uptake in human colorectal cancer HCT116 cells[J].Chin J Radiol Med Prot,2013,33(1):27-31

¹⁸F-FLT细胞摄取率评价结直肠癌HCT116细胞早期放射反应

Investigation of early radiation response by evaluating the capability of ¹⁸F-FLT uptake in human colorectal cancer HCT116 cells

投稿时间：2012-08-04

DOI：10.3760/cma.j.issn.0254-5098.2013.01.007

中文关键词: ¹⁸F-FLT 摄取结直肠癌放疗

英文关键词:¹⁸F-FLT Uptake Colorectal cancer Radiotherapy

基金项目:江苏省"十二五"临床医学重点学科资助;苏州市社会发展项目(SYSD2011095);苏州市科技发展计划项目(SYS201031)

作者	单位	E-mail
朱雅群	215004 苏州大学附属第二医院放疗科
廖岩森	215004 苏州大学附属第二医院放疗科
章斌	苏州大学附属第一医院核医学科
邓胜明	苏州大学附属第一医院核医学科
吴永友	215004 苏州大学附属第二医院普外科
田野	215004 苏州大学附属第二医院放疗科	dryetian@hotmail.com

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中文摘要:

目的观察不同剂量6 MV X射线照射人结直肠癌细胞株HCT116后,体外细胞摄取¹⁸F-FLT的变化,分析¹⁸F-FLT用于早期预测肿瘤放射反应性的可行性。方法测定1.0×10⁵～1.5×10⁶个细胞、培养36、60、84 h条件下,结直肠癌细胞株HCT116的¹⁸F-FLT摄取率。测定细胞经0、2、4、6、8 Gy 6 MV X射线照射后,不同时间点(24、48、72 h)¹⁸F-FLT的细胞摄取率及细胞摄取抑制率;同时测定不同剂量照射后的细胞生长曲线、细胞增殖及细胞周期情况。结果 HCT116细胞对¹⁸F-FLT摄取率达(18.97±1.16)%。¹⁸F-FLT细胞摄取抑制率在照射2、4、6、8 Gy后24 h分别为(32.10±0.02)%、(54.46±0.04)%、(62.74±0.04)%和(65.81±4.81)%;¹⁸F-FLT细胞摄取抑制率与照射剂量呈正相关。¹⁸F-FLT细胞摄取率与细胞周期S期比例呈正相关。结论 ¹⁸F-FLT细胞摄取率可以早期反映人结直肠癌HCT116细胞株对放射的反应性。

英文摘要:

Objective To evaluate the capability of ¹⁸F-FLT uptake and investigate the early radiation response of human colorectal cancer cells HCT116 exposed to 6 MV X-rays.Methods 3.7 kBq ¹⁸F-FLT was added to HCT116 cells with different cell numbers (1.0×10⁵-1.5×10⁶) and cultured with different times (36, 60, 84 h). The ¹⁸F-FLT uptake rate was measured with a γ-counter after exposed to different does of 6 MV X-rays (0, 2, 4, 6, 8 Gy) after 24, 48, and 72 h of irradiation. Then the cell uptake inhibition rate, cell proliferation, and cell cycle phase were measured.Results The uptake rate of ¹⁸F-FLT in HCT116 was (18.97±1.16)%. The ¹⁸F-FLT uptake inhibition rates at 24 h after different does of irradiation (2, 4, 6, 8 Gy) were (32.10±0.02)%, (54.46±0.04)%, (62.74±0.04)%, and (65.81±4.81)%, respectively, which was positively correlated with radiation dose.Conclusions The ¹⁸F-FLT uptake rate of human colorectal cancer HCT116 cells could be used to evaluate the early radiation response.

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