| 刘澜涛,刘建香,高刚,阮健磊,潘艳,朴春南,陈雪,苏旭.不同剂量60Co γ射线对小鼠淋巴细胞及其调节性T细胞亚群的影响[J].中华放射医学与防护杂志,2013,33(1):14-18 |
| 不同剂量60Co γ射线对小鼠淋巴细胞及其调节性T细胞亚群的影响 |
| Changes of lymphocytes and regulatory T cell subset of mice influenced by 60Co γ-ray irradiation |
| 投稿时间：2012-09-24 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.01.004 |
| 中文关键词: 电离辐射 调节性T细胞 表型 |
| 英文关键词:Ionizing radiation Regulatory T cell Phenotype |
| 基金项目:卫生行业科研专项(201002009) |
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| 中文摘要: |
| 目的 分析不同剂量照射对小鼠淋巴细胞及其调节性T细胞亚群数量和表型的影响。方法 用不同剂量60Co γ射线全身照射小鼠,分离胸腺和脾脏中的淋巴细胞,计数各样本细胞数后利用流式细胞仪分析CD4+T细胞和CD4+FOXP3+CD25+Treg细胞亚群的比例。结果 小鼠受照后胸腺细胞和脾脏细胞呈剂量依赖性减少,在照后4 d降至最低(F=118.08、144.01,P<0.05)。较高剂量照射后,胸腺中Treg细胞数随时间逐渐减少,而脾脏中该细胞亚群逐渐恢复;较低剂量照射后,胸腺中Treg细胞数有所增加。与0 Gy组相比较,Treg细胞比例随剂量增加而增加(胸腺中F=5.16、89.44、3.01,P<0.05;脾脏中F=52.02、32.13、27.45,P<0.05)。结论 不同剂量引起淋巴细胞及其Treg细胞亚群的辐射响应不同,其中Treg细胞亚群对较高剂量照射具有显著的辐射抗性,而低剂量照射可能诱导Treg细胞成熟分化或迁移。此外,淋巴细胞及其亚群的不同时间效应,也表明淋巴细胞成熟分化规律的差异。 |
| 英文摘要: |
| Objective To analyze the influence of ionizing radiation on the lymphocytes and its regulatory T cells in mice. Methods Mice were administered with whole body irradiation of γ-rays at different doses, and lymphocytes were separated from thymus and spleen, then the number of total cells were counted and the percentages of CD4+T and CD4+FOXP3+CD25+ Treg lymphocytes were analyzed by using FACS. Results The lymphocyte numbers in thymus and spleen decreased in dose-dependent manner and reached to the minimum at 4 d after irradiation (F=118.08,144.01,P<0.05). Exposure to higher dose(more than 1 Gy) decreased Treg number time-dependently in thymus, however increased it in spleen. On the contrary, exposure to lower dose(less than 0.75 Gy) increased Treg number in thymus. Besides, the percentage of Treg cells increased dose-dependently(in thymus, F=5.16,89.44,3.01,P<0.05;in spleen, F=52.02,32.13,27.45,P<0.05). Conclusions The radiation responses of lymphocytes and their Treg subpopulation vary with the different doses. Treg cells are resistant to high dose irradiation, however, their differentiation could be induced by low dose irradiation. In addition, the different time-dependent responses of lymphocytes and their subpopulation to ionizing radiation indicate the difference of lymphocyte maturation, differentiation and emigration. |
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