| 杨晓云,杜丽,马琼,潘秀颉,蔡金玲,肖凤君,崔玉芳.2 Gy γ射线照射对小鼠调节性T细胞和Th17细胞及其免疫平衡的影响[J].中华放射医学与防护杂志,2013,33(1):6-9 |
| 2 Gy γ射线照射对小鼠调节性T细胞和Th17细胞及其免疫平衡的影响 |
| Effects of 2 Gy γ-irradiation on the number of regulatory T cells and Th17 cells and immune balance in mice |
| 投稿时间：2012-10-15 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.01.002 |
| 中文关键词: γ射线 调节性T细胞 Th17细胞 免疫平衡 |
| 英文关键词:γ-irradiation Regulatory T cells Th17 cells Immune balance |
| 基金项目:国家自然科学基金(30900381,81001219);毫米波国家重点实验室开放课题(K201225) |
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| 中文摘要: |
| 目的 观察2 Gy γ射线照射对小鼠Treg/Th17免疫平衡的影响。方法 50只C57BL/6雄性小鼠随机数字表法分为对照组和照射组,照射组小鼠接受2 Gy γ射线一次性全身照射(剂量率为164.94 Gy/min),对照组行假照射。于照射后1、3、7、14和28 d检测外周血象的变化;流式细胞仪分析外周血、胸腺及脾脏调节性T细胞(Treg细胞)和脾脏Th17细胞数量的改变。结果 受照后小鼠外周血白细胞总数和淋巴细胞数降低(t=8.89～33.54, P<0.05)。小鼠外周血Treg略有升高;胸腺Treg在照后1、3 d显著高于对照组(t=-6.45和-10.59, P<0.05),至28 d明显低于对照组(t=5.34, P<0.05);脾脏Treg在照后1～14 d显著高于对照组(t=-6.82～-3.89, P<0.05)。脾脏Th17细胞于照后1 d出现显著增高,3 d达到最大值(t=-2.42, P<0.05),较脾脏内Treg增长更为明显。Treg/Th17比值在照后1～14 d降低,差异有统计学意义(t=4.02～8.04, P<0.05),导致Treg/Th17平衡模式向Th17方向明显偏移。结论 Treg/Th17免疫平衡失调在辐射所致免疫功能损伤中可能扮演重要角色。 |
| 英文摘要: |
| Objective To observe the effects of 2 Gy γ-ray irradiation on regulatory T cells (Tregs) and Th17 cells and immune balance of mice. Methods A total of fifty C57BL/6 male mice were randomly divided into two groups, the irradiated group exposed to 2 Gy of whole body γ-ray irradiation, and the control group sham-irradiated. At 1, 3, 7, 14 and 28 d after radiation, changes of peripheral haemogram were detected respectively and Tregs in peripheral blood, thymus and spleen and Th17 cells in spleen were analyzed by flow cytometry. Results Compared with control group, the number of peripheral blood white cells (WBC) and lymphocyte in irradiated group reduced significantly post-irradiation (t=8.89-33.54, P<0.05), while the cell number of peripheral CD4+CD25+ Tregs post-irradiation rose but not significantly. Thymic Treg cells increased 1 and 3 d post-irradiation (t=-6.45, -10.59, P<0.05), but reduced 28 d post-irradiation(t=5.34, P<0.05). Splenic Treg cells ascended obviously from 1 to 14 d post-irradiation(t=-6.82-3.89, P<0.05). After irradiation splenic Th17 cells increased at 1 d,and reached the maximal level at 3 d(t=-2.42, P<0.05), more obviously than splenic Treg cells. The reduction of Treg/Th17 ratio from 1 to 14 d post-irradiation disturbed Treg/Th17 balance and made it drift to the direction of Th17(t=4.02-8.04, P<0.05). Conclusions Treg/Th17 imbalance plays an important role in immune injury induced by irradiation. |
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