中华放射医学与防护杂志

董娟聪,单玉兴,邵明龙,等.PLC/PIP2信号通路在辐射诱导NRP1⁺Treg 细胞中的作用机制[J].中华放射医学与防护杂志,2012,32(6):561-564.DONG Juan-cong,SHAN Yu-xing,SHAO Ming-long,et al.Role of PLC/PIP2 signaling pathway in radiation-induced NRP1⁺Treg cells[J].Chin J Radiol Med Prot,2012,32(6):561-564

PLC/PIP2信号通路在辐射诱导NRP1⁺Treg 细胞中的作用机制

Role of PLC/PIP2 signaling pathway in radiation-induced NRP1⁺Treg cells

投稿时间：2012-05-11

DOI：10.3760/cma.j.issn.0254-5098.2012.06.001

中文关键词: 电离辐射 NRP1 调节性T细胞 TGF-β1 PLC/PIP2信号通路

英文关键词:Ionizing radiation Neuropilin-1 Regulatory T-Lymphocytes TGF-β1 PLC/PIP2 signal pathway

基金项目:国家自然科学基金(30870584);国家教育部留学回国人员启动基金(20101201)

作者	单位	E-mail
董娟聪	130021 长春,吉林大学公共卫生学院卫生部放射生物学重点实验室
单玉兴	吉林大学白求恩第一医院骨科
邵明龙	130021 长春,吉林大学公共卫生学院卫生部放射生物学重点实验室
张聪	130021 长春,吉林大学公共卫生学院卫生部放射生物学重点实验室
卢良杰	吉林大学白求恩第一医院骨科
金顺子	130021 长春,吉林大学公共卫生学院卫生部放射生物学重点实验室	shunzij@yahoo.com.cn

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中文摘要:

目的观察X射线照射后小鼠胸腺细胞中CD4+CD25+NRP1⁺Treg细胞数量及TGF-β1分泌量的变化,并探索PLC/ PIP2信号通路在其中的作用机制。方法 36只ICR小鼠按随机数字表法分为假照组、0.5、1.0、2.0、4.0和6.0 Gy的不同剂量组,X射线深部治疗机进行全身照射,于照射后16 h应用流式细胞仪分析小鼠胸腺细胞中CD4+CD25+NRP1⁺Treg细胞的变化,ELISA法检测胸腺细胞TGF-β1含量的变化。EL-4细胞株经PKC激动剂(PMA)、Ca²⁺阻断剂(TMB-8)作用2 h后并经4 Gy X射线照射,于照射后48 h应用流式细胞术及ELISA法分别检测CD4+CD25+NRP1⁺Treg细胞及TGF-β1含量的变化。结果小鼠胸腺细胞中NRP1⁺Treg在0.5 Gy X射线作用后稍有下降,于1.0 Gy降至最低(t=6.96,P<0.01),而后呈剂量依赖性升高,于6.0 Gy升至最高(t=6.70,P<0.01);胸腺细胞TGF-β1在0.5 Gy X射线照射后显著下降(t=12.53,P<0.01),而1.0~4.0 Gy照射后则呈剂量依赖性升高,于6.0 Gy仍保持高水平(t=10.40~15.30,P<0.01)。PMA作用后,与假照组相比,单独PMA组NRP1⁺Treg细胞表达显著降低(t=3.06,P<0.01),而联合照射后表达则明显上调(t=8.27,P<0.01),TGF-β1无论受照与否,其含量均明显降低(t=10.46~39.69,P<0.01);而TMB-8作用后,无论受照与否CD4+CD25+NRP1⁺Treg的表达及TGF-β1的分泌量均显著上调(t=5.53~44.26,P<0.01)。结论高剂量电离辐射可以诱导小鼠胸腺细胞中NRP1⁺ Treg细胞表达并增加TGF-β1的含量,此现象可能与启动PLC/PIP2信号通路有关。

英文摘要:

Objective To explore the role of PLC/PIP2 signal pathway in the changes of mouse thymus CD4+CD25+NRP1⁺Treg and TGF-β1 after different doses of X-ray irradiation Methods 36 ICR mice were randomly divided into 6 groups according to the irradiation doses of 0, 0.5, 1.0, 2.0, 4.0 and 6.0 Gy, respectively. Flow cytometry was used to detect the expression of NRP1⁺Treg, and ELISA was used to detect the expression of TGF-β1 in mouse thymocytes at 16 h post-irradiation. The EL-4 cells were irradiated by X-rays at the dose of 4.0 Gy after co-cultured with the PMA and TMB-8 for 2 hours. Flow cytometry was used to detect the expression of NRP1⁺Treg, and ELISA was used to detect the changes of TGF-β1 at 48 h post-irradiation. Results The NRP1⁺Treg appeared a transient decrease at both 0.5 and 1.0 Gy irradiation and reached its valley value at 1.0 Gy (t=6.96, P<0.01), then showed a dose-dependent increase and reached its peak at 6.0 Gy (t=6.70, P<0.01). The TGF-β1 level decreased after 0.5 Gy X-rays (t=12.53, P<0.01), then increased at a dose-dependent manner and reached its peak at 4.0 Gy (t=10.40-19.56, P<0.01). Compared with the sham-irradiation, NRP1⁺Treg was decreased significantly after PMA treatment (t=3.06, P<0.01), while it was up-regulated significantly after irradiation in the presence of PMA (t=8.27, P<0.01). TGF-β1 was reduced in the presence of PMA with or without irradiation (t=10.46-39.69, P<0.01). NRP1⁺Treg and TGF-β1 were increased significantly after TMB-8 treatment (t=5.53-44.26, P<0.01). Conclusions NRP1⁺Treg cells and TGF-β1 were up-regulated after a high dose radiation, and the PLC/PIP2 signal pathway may participate in the regulation.

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