| 张慧芳,杨彪,党旭红,等.60Coγ射线累积照射对大鼠尿液中小分子代谢物的影响[J].中华放射医学与防护杂志,2012,32(4):358-362.ZHANG Hui-fang,YANG Biao,DANG Xu-hong,et al.Effects of accumulated 60Co γ-ray irradiation on the small molecular metabolites in rats urine[J].Chin J Radiol Med Prot,2012,32(4):358-362 |
| 60Coγ射线累积照射对大鼠尿液中小分子代谢物的影响 |
| Effects of accumulated 60Co γ-ray irradiation on the small molecular metabolites in rats urine |
| 投稿时间：2012-03-15 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.04.006 |
| 中文关键词: 累积照射 尿液代谢成分 核磁共振 主成分分析 |
| 英文关键词:Accumulated irradiation Urinary metabolites Nuclear resonance spectroscopy Principal component analysis |
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| 中文摘要: |
| 目的 探讨60Co γ射线累积照射对大鼠尿液中小分子代谢物的影响。方法 健康成年雄性SD大鼠10只,用60Co γ射线照射大鼠,累积剂量分别为0、0.2、0.4、0.6、0.8和1.0 Gy,连续照射5 d。采用核磁共振(1H NMR)技术,结合主成分分析法(PCA)和偏最小二乘法判别(PLS-DA),分析各累积剂量下大鼠尿液中小分子代谢物的变化。结果 大鼠尿液中的小分子代谢物发生了明显的改变,与对照组相比,0.2~1.0 Gy各组乙酸乙酯的相对含量显著降低(t=29.7~30.7,P<0.05),且其相对含量随剂量增加基本稳定(P>0.05);马尿酸的相对含量增加(t=4.4~21.6,P<0.05),且当累积剂量达到1 Gy时其相对含量显著增加(t=21.6,P<0.05)。三甲胺氮氧化物、牛磺酸、脯氨酸相对含量均比对照组增加(t=3.5~13.4、4.7~11.5和2.9~12.7,P<0.05),且这3种代谢物的相对含量随剂量增加有相同的变化趋势。结论 60Co γ射线累积照射后,尿液中乙酸乙酯、马尿酸、三甲胺氮氧化物、牛磺酸、脯氨酸有可能成为累积照射的辐射损伤标志物。 |
| 英文摘要: |
| Objective To explore the effects of accumulated 60Co γ-ray irradiation on small molecular metabolites in rats urine.Methods Ten healthy male SD rats were irradiated by 60Co γ-rays in 5 days and the accumulated doses were 0, 0.2, 0.4, 0.6, 0.8, 1.0 Gy, respectively. The metabolites in urine of different groups were measured with 1H-NMR combined with principal components analysis (PCA) and partial least square discriminate analysis (PLS-DA). Results The metabolites in rat urine were obviously changed after irradiation. Compared with control group, the amount of acetoacetate decreased after irradiation(t=29.7-30.7,P<0.05), but its relative level was stable when the dose increased (P>0.05). Meanwhile, the relative level of hippuric acid increased(t=4.4-21.6,P<0.05)especially when the accumulated dose was higher than 1 Gy (t= 21.6,P<0.05). The relative level of proline, taurine and trimethylamine-N-oxide increased after irradiation with the same trend(t=3.5-13.4,4.7-11.5,2.9-12.7,P<0.05). Conclusions The acetoacetate, hippuric acid, proline, taurine, and trimethylamine-N-oxide may be applicable for biomarkers of accumulative irradiation on rat. |
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