中华放射医学与防护杂志

田素青,王俊杰,赵勇,等.西妥昔单抗对人舌癌细胞系Tca8113的放射增敏作用[J].中华放射医学与防护杂志,2012,32(4):354-357.TIAN Su-qing,WANG Jun-jie,ZHAO Yong,et al.Radiosensitization of cetuximab on human tongue cancer cell line Tca8113[J].Chin J Radiol Med Prot,2012,32(4):354-357

西妥昔单抗对人舌癌细胞系Tca8113的放射增敏作用

Radiosensitization of cetuximab on human tongue cancer cell line Tca8113

投稿时间：2011-08-08

DOI：10.3760/cma.j.issn.0254-5098.2012.04.005

中文关键词: 西妥昔单抗 Tca8113细胞放射增敏细胞周期凋亡

英文关键词:Cetuximab Tca8113 Radiosensitization Cell cycle Apoptosis

基金项目:

作者	单位	E-mail
田素青	100191 北京大学第三医院肿瘤中心
王俊杰	100191 北京大学第三医院肿瘤中心	doctornangjunjie@yahoo.com.cn
赵勇	中国科学院动物研究所生物膜国家重点实验室
江萍	100191 北京大学第三医院肿瘤中心
姜玉良	100191 北京大学第三医院肿瘤中心
王皓	100191 北京大学第三医院肿瘤中心
孟娜	100191 北京大学第三医院肿瘤中心

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中文摘要:

目的探讨西妥昔单抗(C225)对人舌癌Tca8113细胞系的放射增敏作用。方法体外培养人舌癌Tca8113细胞系,C225组用西妥昔单抗(C225)100 nmol/L处理后,6 MV X射线不同剂量 (0、2、4、6、8和10 Gy) 照射C225组和单纯照射组,照射后用细胞计数法及四甲基偶氮唑盐比色法(MTT)检测细胞增殖率,集落形成实验计算克隆数,采用流式细胞仪检测细胞周期、细胞凋亡。结果不同照射剂量时C225+照射组的细胞生长抑制率均高于单纯照射组(t=-15.6~-3.0,P<0.05);C225+照射组的放射生物学参数值(D₀、D_q、N、SF₂)较单纯照射组低。C225+照射组的SER为1.353;C225+照射组的G₀/G₁期细胞比例在4、6、8 Gy时均高于单纯照射组(t=-7.64、-7.89、-4.78,P<0.05)。随着照射剂量升高,C225＋照射组和单纯照射组的细胞早期凋亡率皆先升高后下降,4 Gy时两组差异最大[(7.96±0.36)%和(4.13±0.29)%,t=-12.75,P<0.01]。结论 C225对人舌癌Tca8113细胞系具有放射增敏作用。C225可能通过G₀/G₁ 期阻滞和诱导凋亡来增加人舌癌Tca8113细胞系的放射敏感性。

英文摘要:

Objective To investigate the mechanism of radiosensitization by cetuximab (C225) on human tongue cancer Tca8113 cell line in vitro.Methods Tca8113 cell line with and without C225 treatment received 6 MV X-ray irradiation of different doses (0, 2, 4, 6, 8 and 10 Gy). Cell proliferation, cell-cycle distribution and clonogenic survival were analyzed through cell counting, MTT, colony formation assay, and flow cytometry, respectively. Results After irradiation of different doses, the growth inhibition rates in C225 group were higher than control (t=-15.6-3.0,P<0.05), the radiobiological parameters (D₀, D_q, N, and SF₂) in C225 group were lower than control so that SER of C225 group was 1.353, and the proportions of G₀/G₁ cells in C225 group were higher than control (t=-7.64,-7.89,-4.78,P<0.05) at 4,6,8 Gy. When the irradiation doses increased, the early phase apoptosis in both groups increased at first and then decreased with the maximum difference at 4 Gy [(7.96±0.36)% in C225 group and (4.13±0.29)% in control group,t=-12.75, P<0.01]. Conclusions C225 has radiosensitization effect on Tca8113 cell line, possible through G₀/G₁ arrest and induction of apoptosis.

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