| 王芹,王颖嫒,宋力,等.白介素21基因联合放射对子宫颈癌HeLa细胞的协同抑制作用[J].中华放射医学与防护杂志,2012,32(4):350-353.WANG Qin,WANG Ying-ai,SONG Li,et al.Synergic effect of human IL-21 gene transfer combined with γ-ray irradiation on the growth of cervical carcinoma HeLa cells[J].Chin J Radiol Med Prot,2012,32(4):350-353 |
| 白介素21基因联合放射对子宫颈癌HeLa细胞的协同抑制作用 |
| Synergic effect of human IL-21 gene transfer combined with γ-ray irradiation on the growth of cervical carcinoma HeLa cells |
| 投稿时间：2011-12-15 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.04.004 |
| 中文关键词: IL-21基因 电离辐射 子宫颈癌细胞 增殖抑制 |
| 英文关键词:Interleukin-21 gene Irradiation Cervical carcinoma cell Growth inhibition |
| 基金项目:天津市自然科学基金(09JCYBJC09300) |
|
| 摘要点击次数: 3992 |
| 全文下载次数: 2861 |
| 中文摘要: |
| 目的 探讨外源性IL-21基因表达联合放射对子宫颈癌HeLa细胞的协同抑制作用。方法 将构建好的重组腺病毒Ad-IL-21转染人胚肾293A细胞,进行病毒扩增,采用TCID50方法测定腺病毒的滴度。将扩增的Ad-IL-21腺病毒于体外转染HeLa细胞,转染后进行6 Gy 137Cs γ射线照射,利用随机数字表法分为5组,空白对照组不加任何处理,含LacZ基因、但不含IL-21基因的对照腺病毒为Ad-LacZ对照组,Ad-IL-21组,单纯照射组以及联合处理组,观察HeLa细胞的细胞生长曲线、细胞周期、细胞凋亡、IL-21基因和蛋白表达的变化。结果 Ad-IL-21重组腺病毒在293A细胞中扩增成功,获得了高滴度的病毒颗粒,滴度为9×1010 pfu/ml。Ad-IL-21组、单纯照射组和联合处理组中,HeLa细胞的生长均明显受到抑制,其中转染后96 h,与Ad-IL-21组和单纯照射组比,联合处理组HeLa细胞的抑制效应最显著 (F=85.26、72.98,P<0.05)。联合处理组HeLa细胞发生G1期阻滞,S期细胞数量减少(F=36.69、34.83,P<0.05),凋亡细胞数量增加(F=28.23、25.57,P<0.05)。联合处理组HeLa细胞中IL-21基因表达量分别是Ad-IL-21组和空白对照组的1.54倍和2.43倍(F=22.31、36.65,P<0.05);蛋白表达量分别是Ad-IL-21组和空白对照组的1.62倍和2.31倍(F=27.36、35.86,P<0.05)。结论 IL-21基因联合放射对子宫颈癌细胞的抑瘤作用具有协同效应,有效地抑制肿瘤细胞的生长。 |
| 英文摘要: |
| Objective To study the combined effect of interleukin-21 gene transfer and ionizing radiation on the growth of cervical carcinoma HeLa cells.Methods Previously constructed Ad-IL-21 gene was amplified by infecting 293A cells and the titer was measured by TCID50 method. HeLa cells were transfected with Ad-IL-21 and then irradiated with 6 Gy 137Cs γ-rays. The cells were divided into 5 groups,including blank control,Ad-LacZ group,Ad-IL-21 group,radiation group and Ad-IL-21 combined with radiation group (combination group). The cell growth, cell cycle, apoptosis, and the expressions of IL-21 gene and protein in HeLa cells were detected. Results Ad-IL-21 was successfully amplified and the titer of Ad-IL-21 was 9×1010 pfu/ml. Compared with Ad-IL-21 group and radiation group,the cell growth of combination group was significantly inhibited at 96 h after transfection (F=85.26,72.98,P<0.05). The cells in combination group were arrested in G1 phase and decreased at S phase(F=36.69,34.83, P<0.05), while the cellular apoptosis increased markedly(F=28.23,25.57, P<0.05). The gene expression of IL-21 in the combination group was 1.54- and 2.43-fold of Ad-IL-21 group and blank control group, respectively(F=22.31,36.65, P<0.05), while the protein expression of IL-21 in the combination group was 1.62-fold and 2.31-fold of Ad-IL-21 group and blank control group, respectively(F=27.36,35.86, P<0.05). Conclusions Ad-IL-21 gene transfection combined with radiation has synergic effect on the inhibition of cervical carcinoma cell growth. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|