| 王慧,王鑫,庞得全,范玉敏,胡雷,陈婕,苏华坤,姚林.安多霖预防小鼠放射性肺损伤的初步实验研究[J].中华放射医学与防护杂志,2012,32(3):278-281 |
| 安多霖预防小鼠放射性肺损伤的初步实验研究 |
| Experimental study of preventive effect of Anduolin (ADL) on radiation-induced lung injury in mice |
| 投稿时间：2011-08-22 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.014 |
| 中文关键词: 放射性肺损伤 安多霖 羟脯氨酸 转化生长因子-β1 |
| 英文关键词:Radiation-induced lung injury Anduolin Hydroxproline Transforming growth factor-β1 |
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| 中文摘要: |
| 目的 观察安多霖(ADL)对小鼠放射性肺损伤的防治作用。方法 成年雄性昆明种小鼠180只,随机数字表法分为正常对照、照射对照和ADL低、中、高剂量及地塞米松治疗6组,每组30只。后5组小鼠用6 MV X射线一次胸部照射20Gy,均于照射前1 d开始灌胃给药,连续6周,ADL剂量分别为2、4和8g/kg,地塞米松为0.12 mg·kg-1·d-1。照射后观察各组小鼠一般状况,呼吸频率;在第2、4和6周时取肺组织做肺系数测定,同时测定小鼠肺羟脯氨酸含量及转化生长因子-β1(TGF-β1)的含量;HE染色观察肺组织病理改变。结果 ADL低剂量组与照射对照组相比,呼吸频率、肺羟脯氨酸及TGF-β1的含量差异无统计学意义。ADL中、高剂量组和地塞米松组与照射对照组相比,呼吸频率减慢、肺羟脯氨酸及TGF-β1的含量降低差异有统计学意义(F=2.668~161.646,P<0.05)。HE染色观察肺组织病理改变显示,ADL中、高剂量组和地塞米松组与照射对照组相比,肺组织充血、水肿及肺泡壁增厚程度减轻。结论 安多霖可以降低胸部照射后小鼠肺羟脯氨酸及TGF-β1的含量,减轻小鼠放射性肺损伤程度,对放射性肺损伤具有一定的防治作用。 |
| 英文摘要: |
| Objective To observe the preventive effect of Anduolin(ADL) on radiation-induced lung injury in mice.Methods Totally 180 of Kunming mice were randomly classified into six groups: normal control group (N),irradiation control group (R), irradiation plus low dose ADL group (L), irradiation plus middle dose ADL group (M), irradiation plus high dose ADL group (H), and irradiation plus Dexamethasone group (D). The mice except group N were irradiated with 20 Gy of 6 MV X-rays on whole lung. The mice in group L, M and H were given with ADL 1 d before irradiation and continued for 6 weeks after irradiation.At 2, 4 and 6 weeks after irradiation, the general situation and the lung pathological changes of mice were observed. The lung wet weight, collagen contents of the whole lung tissue, hydroxproline concentration, and TGF-β1 expression in the lung were also detected.Results Compared to the group R, the mice breathing rate, hydroxproline concentration, and TGF-β1 expression in the group L were not significantly changed. While in the groups M, H and D, the breathing rate, the generation of hydroxproline and the expression of TGF-β1 were decreased significantly (F=2.668-161.646,P<0.05). In addition, ADL alleviated the pathological changes on radiation-induced lung injury in mice.Conclusions ADL might have the preventive effect on radiation-induced lung injury in mice. |
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