| 乔俏,李光.电离辐射诱导HIF-1α-Survivin通路在人淋巴瘤细胞中的活化[J].中华放射医学与防护杂志,2012,32(3):274-277 |
| 电离辐射诱导HIF-1α-Survivin通路在人淋巴瘤细胞中的活化 |
| Ionizing radiation activates the HIF-1α-Survivin pathway in human lymphoma cells |
| 投稿时间：2011-11-11 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.013 |
| 中文关键词: 淋巴瘤 乏氧诱导因子-1α 生存素 电离辐射 |
| 英文关键词:Lymphoma Hypoxia inducible factor-1α Survivin Ionizing radiation |
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| 中文摘要: |
| 目的 研究电离辐射对人非霍奇金淋巴瘤细胞中HIF-1α-Survivin通路活化状态的影响,探讨恶性淋巴瘤放射抗性的机制。方法 采用Western blot方法检测辐射后3种淋巴瘤细胞中HIF-1α和Survivin蛋白的表达水平,观察应用HIF-1α抑制剂Echinomycin及转染反义HIF-1α siRNA对Survivin蛋白及mRNA表达的影响。结果 在人非霍奇金淋巴瘤细胞中存在HIF-1α和Survivin蛋白的表达,X射线照射后10~20 h出现HIF-1α蛋白表达增加,照射后24 h Survivin蛋白表达增加,与对照组比较差异有统计学意义(t=7.53~31.31,P<0.01)。与单纯照射组比较,采用HIF-1α抑制剂预处理肿瘤细胞后, Survivin蛋白表达下降,且具有药物浓度依赖性(t=7.21~32.81,P<0.01)。而转染反义HIF-1α siRNA后,照射未诱导产生survivin mRNA和蛋白表达增加。结论 电离辐射能够活化人非霍奇金淋巴瘤细胞中的HIF-1α-Survivin通路,可能与肿瘤的放射抗性有关。 |
| 英文摘要: |
| Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1α specific antagonist or HIF-1α siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC), a HIF-1α specific inhibitor, or transfection of HIF-1α siRNA. Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR, respectively.Results After irradiation, the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t=7.53-31.31,P<0.01) in three lymphoma cell lines. Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t=7.21-32.81,P<0.01). However, radiation failed to induce up-expression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusions HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation, which might be involved in the cell radioresistance. |
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