| 孙克康,赵琳,徐加英,等.电离辐射及顺铂对人肺癌细胞中TOB1基因表达的影响[J].中华放射医学与防护杂志,2012,32(3):263-265.SUN Ke-kang,ZHAO Lin,XU Jia-ying,et al.Effect of ionizing radiation and cisplatin on the expression of TOB1 in human lung cancer cell lines[J].Chin J Radiol Med Prot,2012,32(3):263-265 |
| 电离辐射及顺铂对人肺癌细胞中TOB1基因表达的影响 |
| Effect of ionizing radiation and cisplatin on the expression of TOB1 in human lung cancer cell lines |
| 投稿时间：2011-08-18 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.010 |
| 中文关键词: 肺癌 电离辐射 顺铂 erbB2转录因子1 |
| 英文关键词:Lung cancer Ionizing radiation Cisplatin Transducer of erbB2.1 |
| 基金项目:教育部博士点新教师基金(K512602110) |
|
| 摘要点击次数: 4041 |
| 全文下载次数: 2597 |
| 中文摘要: |
| 目的 探讨电离辐射及其联合顺铂对人肺腺癌细胞株SPCA-1、LTEP-α-2中TOB1基因表达的影响。方法 体外培养人肺腺癌SPCA-1、LTEP-α-2细胞,用X射线一次性照射,细胞吸收剂量为2、4、6、8和10 Gy,源靶距为100 cm,吸收剂量率为200 cGy/min,在6 Gy X射线照射后6、12、18和24 h取样; X射线和顺铂联合对TOB1表达的研究设立单纯照射组(6 Gy)、顺铂处理组(20 mol/L 顺铂)和联合处理组(6 Gy +20 mol/L 顺铂);上述实验同时设立未处理的对照组。采用RT-PCR和Western blot法检测TOB1 mRNA及蛋白表达水平的变化。结果 SPCA-1、LTEP-α-2细胞在不同剂量的X射线照射后24 h,其TOB1蛋白及mRNA表达量均明显增加(t=8.25~24.48, P<0.05);不同时间检测提示,细胞在X射线照射后6 h,TOB1蛋白及mRNA的表达量即开始上升,并持续到照射后24 h(t=14.23~15.82, P<0.05);联合处理组TOB1的表达量均明显高于单纯照射组、顺铂处理组和对照组(t=4.67~13.67, P<0.05)。结论 TOB1基因有可能作为临床肺癌放化疗靶基因,对临床放化疗疗效的判断具有一定的应用潜力。 |
| 英文摘要: |
| Objective To explore the effect of ionizing radiation and cisplatin on the expression of Transducer of erbB2,1 (TOB1) in human lung adenocareinoma SPCA-1 and LTEP-α-2 cell lines.Methods SPCA-1 and/or LTEP-α-2 cells were respectively irradiated with 2,4,6,8,and 10 Gy X-rays generated by a linear accelerator (with the source skin distance of 100 cm and dose rate of 200 cGy/min). The cell molecular samples were subtracted at 6,12,18,and 24 h after 6 Gy irradiation. For X-rays and cisplatin combination treatment, cells were divided into radiation group (6 Gy X-rays), cisplatin group (20 mol/L cisplatin), and combination group (6 Gy X-rays+20 mol/L cisplatin). SPCA-1 and LTEP-α-2 cells without any treatment were used as control group. The relative levels of TOB1 mRNA and protein expression were detected by rigorously controlled semi-quantitative RT-PCR and Western blot analysis with quantitation of the mRNA/protein bands by densitometry.Results The expression level of TOB1 was significantly increased in both mRNA and protein level after radiation exposure (t=8.25-24.48, P<0.05). For time-dependent induction of TOB1, the expression was significantly increased 6 h after X-rays exposure (t=14.23-15.82, P<0.05). More significant increase of TOB1 expression was identified after the combination treatment of X-rays and cisplatin, compared to that of the radiation and/or cisplatin treatment alone (t=11.21-13.67, P<0.05).Conclusions Ionizing radiation and cisplatin could upregulate the expression of TOB1 in lung cancer cells in both mRNA and protein levels. TOB1 may be a molecular target for ionizing radiation and cisplatin treatment in lung cancer and it may have potential implication in evaluating the curative efficiency of chemo-and radio-therapy. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|