| 朱小东,黄诗婷,曲颂,苏芳,郭亚,王金子.鼻咽癌细胞辐射抗拒的比较蛋白质组学分析[J].中华放射医学与防护杂志,2012,32(3):245-248 |
| 鼻咽癌细胞辐射抗拒的比较蛋白质组学分析 |
| Comparative proteomic analysis on radioresistant nasopharyngeal carcinoma cell |
| 投稿时间：2011-05-10 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.006 |
| 中文关键词: 鼻咽癌 辐射抵抗 比较蛋白质组学 |
| 英文关键词:Nasopharyngeal carcinoma Radioresistance Comparative proteomics |
| 基金项目:国家自然科学基金(30860329);广西大型仪器协作共用网测试项目(851-2009-055);广西2010年研究生教育创新计划项目(2010105981002M215) |
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| 中文摘要: |
| 目的 通过分离并鉴定具有不同辐射抗拒的人鼻咽癌细胞株CNE-2R与CNE-2的差异表达蛋白,探讨与鼻咽癌细胞辐射抗拒相关的分子机制。方法 分别提取鼻咽癌辐射抗拒细胞株CNE-2R及其亲本细胞株CNE-2的总蛋白,采用双向凝胶电泳分离,经软件分析识别差异表达蛋白点,应用基质辅助激光解析电离飞行时间串联质谱技术(MALDI-TOF-MS)鉴定差异蛋白质。结果 两种不同辐射敏感性的鼻咽癌细胞株CNE-2R和CNE-2中,共筛选出差异表达明显的蛋白质点有32个,其中11个蛋白质被鉴定成功,在CNE-2R中上调的蛋白有3个,下调的蛋白有8个。结论 不同辐射敏感性的鼻咽癌细胞的差异表达蛋白,主要涉及调节凋亡、DNA损伤与修复、细胞周期调控、RNA转录、细胞信号转导、细胞骨架组成及辐射应激反应等多个方面。 |
| 英文摘要: |
| Objective To discover radioresistance-associated proteins by performing comparative proteomic analysis on nasopharyngeal carcinoma cell lines.Methods The total proteins were extracted from radioresistant human nasopharyngeal carcinoma cell line CNE-2R and its parental cell line CNE-2, respectively. These proteins were separated by high quality two-dimensional polyacrylamide gel electrophoresis (2-DE) and then the 2-DE profiles were screened for differentially expressed protein spots by the Image Master 5.0 software. Those spots were identified by a matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.Results 32 significantly differentially expressed protein spots were screened in two different radiosensitivity cell lines and 11 proteins were identified by tandem mass spectrometry, among which 3 proteins were up-regulated in radioresistant human nasopharyngeal carcinoma cell line CNE-2R and the other 8 proteins were down-regulated.Conclusions The differentially expressed proteins of nasopharyngeal carcinoma cells with different radiosensitivity were mainly involved in apoptosis regulation, DNA damage and repair, cell cycle regulation, RNA transcription, cell signaling, cytoskeleton formation and radiation stress responses. |
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