| 赵琳,孙克康,申琳棱,焦旸,徐加英,樊赛军.表没食子儿茶素没食子酸酯对人鼻咽癌CNE-1细胞辐射敏感性的影响[J].中华放射医学与防护杂志,2012,32(3):236-240 |
| 表没食子儿茶素没食子酸酯对人鼻咽癌CNE-1细胞辐射敏感性的影响 |
| Effect of epigallocatechin-3-gallate on radiosensitivity of CNE-1 cells |
| 投稿时间：2011-10-24 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.004 |
| 中文关键词: 表没食子儿茶素没食子酸酯 鼻咽癌 辐射敏感性 细胞周期 凋亡 |
| 英文关键词:EGCG Nasopharyngeal carcinoma Radiosensitivity Cell cycle Apoptosis |
| 基金项目:国家科学自然研究基金面上项目(81071906;81172127);教育部"长江学者和创新团队发展计划资助"项目(IRT0849);江苏高校优势学科建设工程资助项目(PAPD) |
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| 中文摘要: |
| 目的 探讨表没食子儿茶素没食子酸酯(EGCG)对人鼻咽癌CNE-1细胞辐射敏感性的影响及相关机制。方法 将细胞分为对照组、EGCG单独处理组、UVC或X射线单独照射组,EGCG联合UVC或X射线照射组。四甲基偶氮唑盐(MTT)比色法检测不同浓度EGCG处理24、48和72 h对CNE-1细胞生长的影响,以及EGCG联合紫外线(UVC)及X射线对CNE-1细胞生长的影响。克隆形成实验检测EGCG对CNE-1细胞的放射增敏作用。流式细胞术检测细胞周期变化。Annexin V-FITC法检测细胞凋亡。Western blot法检测相关蛋白表达水平。结果 EGCG可抑制CNE-1细胞的生长,并有剂量-效应关系(r = 0.817)和处理时间-效应关系(r = 0.364)。EGCG在低剂量50 μmol/L预处理2 h,可增加CNE-1细胞对UVC和X射线的辐射敏感性。相比于单独照射组,EGCG联合UVC照射组细胞的S期阻滞消失(t=18.68,P<0.05),sub-G1峰的比例则明显增高(t=6.67,P<0.05)。而且,Annexin V-FITC检测结果揭示,EGCG联合UVC照射组的细胞中早期凋亡细胞比例显著增加(t=10.28,P<0.05)。但与单独照射组细胞相比,EGCG联合X射线照射组细胞的S期及G2/M期阻滞更明显(t=7.11和6.99,P<0.05),无明显的sub-G1峰出现。EGCG联合UVC照射可使凋亡相关蛋白Bax表达增加(t=5.42,P<0.05),Caspase-3表达增加(t=4.14,P<0.05),Bcl-2的表达变化并不明显(t=1.85,P>0.05),而其联合X射线对于细胞周期相关蛋白 Cdc2 p34表达的影响不大(t=1.04,P>0.05)。结论 EGCG可抑制鼻咽癌CNE-1细胞的生长。EGCG与UVC联合损伤作用与促进细胞发生早期凋亡相关,涉及到凋亡相关蛋白Bax和Caspase-3;而EGCG和X射线的联合损伤作用可能与周期阻滞相关。 |
| 英文摘要: |
| Objective To investigate the effect of EGCG on the radiosensitivity of human nasopharyngeal carcinoma CNE-1 cells.Methods CNE-1 cells were divided into four groups: control, EGCG treatment, UVC or X-ray exposure, and EGCG combined with UVC or X-rays. After treatment with different concentrations of EGCG for 24, 48 and 72 h and UVC or X-rays, cell growth was determined with MTT assay, cell survival was measured with clonogenic assay, cell cycle was detected with flow cytometry, cell apoptosis was detected by the annexin V-FITC cell apoptosis kit, and protein expression was assayed by Western blot.Results EGCG inhibited cell growth in a dose-and time-dependent manner(r = 0.817 and 0.364). Compared with UVC or X-ray irradiation alone, the radiosensitivity of CNE-1 cells was enhanced by 2 h pre-treatment of 50 μmol/L EGCG,which disrupted S phase arrest caused by UVC(t =18.68,P<0.05)and increased the population of S and G2/M arrest caused by X-rays(t =7.11 and 6.99,P<0.05). UVC could cause a significant increase of sub-G1 population(t =6.67,P<0.05)and Annexin V-FITC assay indicated apoptosis was further elevated by EGCG(t =10.28,P<0.05). However, no significant induction of apoptosis was observed in the cells either irradiated with X-rays alone or combinationly treated with EGCG and X-rays. The combination treatment of EGCG and UVC significantly increased the expression of Bax and Caspase-3 proteins, but failed to affect Bcl-2 protein expression.Conclusions EGCG enhances the growth inhibition of CNE-1 cells caused by UVC or X-rays, which is relevant to apoptosis induction or cell cycle arrest. |
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