| 宋娜玲,孟爱民,王月英,吴红英,贺欣,李德冠,王小春,张恒,路璐.17aα-D-高炔雌二醇-3-乙酯联合γ射线对黑色素瘤B16生长的抑制作用[J].中华放射医学与防护杂志,2012,32(3):233-235 |
| 17aα-D-高炔雌二醇-3-乙酯联合γ射线对黑色素瘤B16生长的抑制作用 |
| Inhibitory effect of 17aα-D-homo-ethynylestradiol-3-acetae combined with irradiation on melanoma B16 growth |
| 投稿时间：2011-12-22 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.003 |
| 中文关键词: 17aα-D-高炔雌二醇-3-乙酯 B16黑色素瘤 放射治疗 肿瘤抑制率 |
| 英文关键词:17aα-D-homo-ethynylestradiol-3-acetae B16 melanoma Radiation therapy Tumor inhibition efficiency |
| 基金项目:国家自然科学基金(81072237);天津市自然科学基金重点项目(11JCZDJC19100) |
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| 中文摘要: |
| 目的 建立移植性黑色素瘤B16小鼠肿瘤动物模型,观察17aα-D-高炔雌二醇-3-乙酯抗肿瘤效应及其对免疫器官的影响,探讨合用137Cs γ射线是否具有抑瘤增效的作用。方法 将80只IRM-2荷瘤小鼠用完全随机法分为对照组、照射组、17aα-D-高炔雌二醇-3-乙酯低、中、高(5、7.5、10 mg/kg)药物组及药物联合照射组,每组10只。药物组与药物联合照射组对应性腹腔注射相同剂量17aα-D-高炔雌二醇-3-乙酯, 1次/d,连续7 d。药物联合照射组和照射组于给药的第4天进行全身1 Gy γ射线照射,1次/d,连续5 d。观察各组小鼠肿瘤抑制率及骨髓有核细胞数、胸腺与脾指数指标,比较各组间的差别。结果 17aα-D-高炔雌二醇-3-乙酯各剂量组瘤重明显小于对照组(t=4.58、9.07、6.67,P<0.001),联合137Csγ射线后能提高抑瘤效果与对照组比较(t=8.09、10.35、6.71、P <0.05),17aα-D-高炔雌二醇-3-乙酯各组骨髓有核细胞数、胸腺指数和脾指数均高于对照组,骨髓有核细胞数与对照组比较差异有统计学意义(t=2.638、3.803、2.841,P<0.01),高剂量组的胸腺指数和脾指数与对照组比较差异有统计学意义(t=2.599、2.540,P <0.01),结论 17aα-D-高炔雌二醇-3-乙酯抑制小鼠黑色素瘤B16的生长,对造血和免疫系统可能有保护或促进恢复的作用。 |
| 英文摘要: |
| Objective To investigate the antitumor effect of 17aα-D-homo-ethynylestradiol-3-acetae on the mice transplanted with melanoma (B16) tumor cells, and to explore the possible synergistic effect with irradiation.Methods IRM-2 mice transplanted with B16 cells were randomly classified into control group, irradiation group, 17aα-D-homo-ethynylestradiol-3-acetae drug (high dose, medium dose, low dose) groups, and drug and irradiation combination group. Mice in drug group and the combination group were intraperitoneally injected with 5, 7.5, and 10 mg/kg drug for 7 days. Mice in the irradiation and combination group received 1 Gy total body irradiation at 4 d after drug injection and then once a day for 5 days. The tumor inhibition efficiency, the number of bone marrow cells, thymus indices, and spleen indices were evaluated.Results Tumor weights in each drug group were significantly lower than those of the control(t=4.58,9.07,6.67,P <0.05). Drug combinated with 137Csγ-rays enhanced the antitumor effect so that the tumor weights in the combination group were significantly decreased (t=8.06,10.35,6.71,P <0.05) in comparison with the control groups. Moreover, the numbers of marrow nucleated cells, thymus index and spleen index in the drug group were higher than those in the control group(t=2.64,3.80,2.84,P <0.05).Conclusions 17aα-D-homo-ethynylestradiol-3-acetae can inhibit cell growth of Bl6 melanoma in mice and may also have radioprotective effect on the hematopoietic system and immune system of mice. |
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