| 宋传军,孟星君,谢玲,等.血红素氧化酶-1治疗放射性皮肤损伤的疗效观察[J].中华放射医学与防护杂志,2012,32(3):230-232.SONG Chuan-jun,MENG Xing-jun,XIE Ling,et al.Effect of heme oxygenase-1 on radiation-induced skin injury[J].Chin J Radiol Med Prot,2012,32(3):230-232 |
| 血红素氧化酶-1治疗放射性皮肤损伤的疗效观察 |
| Effect of heme oxygenase-1 on radiation-induced skin injury |
| 投稿时间：2011-07-27 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.002 |
| 中文关键词: 血红素氧化酶 腺病毒 放射性皮肤损伤 基因治疗 |
| 英文关键词:Heme oxygenase Adenovirus Irradiation-induced skin injury Gene therapy |
| 基金项目:国家自然科学基金(81102078) |
|
| 摘要点击次数: 3946 |
| 全文下载次数: 2757 |
| 中文摘要: |
| 目的 探讨介导血红素氧化酶-1(heme oxygenase,HO-1)的腺病毒在治疗急性放射性皮肤损伤中的作用。方法 选取33只雄性SD大鼠,电脑数字表法随机分为3组,磷酸盐缓冲液(PBS)对照组、介导绿色荧光蛋白基因的腺病毒(Ad-EGFP)组及介导HO-1基因的腺病毒(Ad-HO-1)组,每组11只。每组取3只大鼠,用于检测腺病毒目的基因的表达。用直线加速器产生的40 Gy负电子线照射各组余下大鼠臀部皮肤。照射后立即分别皮下注射PBS、Ad-EGFP或Ad-HO-1。采用半定量方法对皮肤损伤评分,每周评定2次,观察7周。结果 注射Ad-HO-1的皮肤真皮层内见HO-1表达强阳性。照射14 d后,Ad-HO-1组皮肤损伤明显低于PBS对照组和Ad-EGFP组(q=0.000~0.030,P<0.05)。结论 HO-1能显著减轻急性放射性皮肤损伤,表明Ad-HO-1可以用于治疗放射性皮肤损伤。 |
| 英文摘要: |
| Objective To investigate the effect of heme oxygenase-1(HO-1) on the acute radiation-induced skin injury by gene transfer.Methods Thirty-three male SD rats were randomly divided into three groups as PBS-injected group, Ad-EGFP-injected group and Ad-HO-1-injected group (n=11). In each group, three rats were used for determining the expression of target gene and the other rats were irradiated on the buttock skin with 40 Gy electron beam generated by a linear accelerator. Immediately after irradiation, rats were administered with a subcutaneous injection of PBS, Ad-EGFP or Ad-HO-1, respectively. Subsequently, the skin reactions were measured twice a week using the semi-quantitative skin injury scale.Results The strong positive expression of HO-1 was observed in subcutaneous dermal tissue after injection of Ad-HO-1.Compared to the PBS-injected group or the Ad-EGFP-injected group, a significant mitigation of skin injury was observed in Ad-HO-1-injected mice 14 d after irradiation (q=0.000－0.030, P<0.05).Conclusions HO-1 could significantly mitigate radiation-induced acute skin injury and Ad-HO-1 could be used to treat radiation-induced skin injury. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|