| 邱晶,朱国英,顾淑珠,陈晓,邵春林.137 Cs γ射线对成骨细胞增殖、分化和矿化的影响[J].中华放射医学与防护杂志,2012,32(2):191-195,203 |
| 137 Cs γ射线对成骨细胞增殖、分化和矿化的影响 |
| Effects of 137Cs γ-rays on proliferation, differentiation and mineralization of osteoblastic cells in vitro |
| 投稿时间：2011-09-22 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.02.021 |
| 中文关键词: γ射线|成骨细胞|增殖|分化|矿化|基因表达 |
| 英文关键词:Gamma irradiation|Osteoblastic cells|Proliferation|Differentiation|Mineralization|Gene expression |
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| 摘要点击次数: 3165 |
| 全文下载次数: 1992 |
| 中文摘要: |
| 目的 探讨137Cs γ射线对成骨细胞形态、增殖、分化、矿化及细胞因子的影响及分子机制。方法 将成骨细胞分为对照组(0 Gy)及0.5、1.0、2.0和5.0 Gy组,分别接受137Cs γ射线照射,倒置相差显微镜观察各组细胞形态,MTT法测定细胞增殖,PNPP法检测碱性磷酸酶(ALP)活性,茜素红染色法观察矿化功能,RT-PCR半定量检测ALP、骨钙素(OC)、Ⅰ型胶原(collagen Ⅰ)、护骨素(OPG)和NF-κB 受体活化因子配体(RANKL)等基因mRNA表达量。结果 1.0 Gy以上照射组抑制成骨细胞增殖(t = 6.197~18.677,P<0.05);2.0 Gy以上照射组致细胞数量减少,折光性减低,细胞间突起连接减少,并可抑制细胞ALP 活性和矿化能力(t=2.790~21.374,P<0.05)。ALP和OC 基因mRNA表达量在0.5 Gy以上剂量照射即明显下调(t=3.563~16.508,P<0.05),OPG、OPG/RANKL在5.0 Gy剂量时表达明显下调(t =12.942、 4.954,P<0.05),Ⅰ型胶原和RANKL基因mRNA的表达未见明显改变。结论 137Cs γ射线照射致成骨细胞形态改变,增殖、分化和矿化能力下降,ALP、OC、OPG等相关基因表达下调,OPG/RANKL通路可能是大剂量电离辐射时骨损伤的主要作用途径之一。 |
| 英文摘要: |
| Objective To evaluate the effect of gamma irradiation on the proliferation, differentiation, and mineralization of murine osteoblastic cells, and to investigate the related molecular mechanism. Methods Osteoblastic cells were irradiated by different doses (0, 0.5, 1.0, 2.0, 5.0 Gy) of 137Cs γ-rays. Cell morphology was observed with a microscopy, cell viability was analyzed by MTT assay, and ALP activity was analyzed by the methods of enzyme histochemistry and PNPP. Meanwhile, gene expressions of ALP, osteocalcin (OC), collagen Ⅰ, osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) were measured by semi-quantified RT-PCR. Results Cell viability decreased with the radiation doses over 1.0 Gy(t=6.197-18.677, P<0.05). After radiation with a dose over 2.0 Gy, the cell number and the junctions of cell protrusions decreased, the cells had low refractivity and the activity and mineralization ability of ALP were also inhibited (t=2.790-21.374, P<0.05). In addition, the expressions of ALP and OC mRNA were down-regulated significantly (t=3.563-16.508, P< 0.05) when the radiation dose was higher than 0.5 Gy, and the expressions of OPG, OPG/RANKL mRNA were down-regulated (t=12.942, 4.954, P<0.05) at 5 Gy. But the expressions of collagen Ⅰ and RANKL mRNA were not affected by irradiation. Conclusions The osteoblastic cells were significantly influenced by γ-irradiation, including morphological changes, inhibition of cell proliferation, differentiation and mineralization ability. Meanwhile, mRNA expressions of ALP and OC were down-regulated. OPG/RANKL may be a main pathway of osteoblastic cell damage under high dose radiation. |
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