| 罗庆斌,陈肖华,董波,姜合作,李峰生,李博.小剂量γ射线全身照射对小鼠脑组织氨基酸类神经递质含量的影响[J].中华放射医学与防护杂志,2012,32(2):179-181 |
| 小剂量γ射线全身照射对小鼠脑组织氨基酸类神经递质含量的影响 |
| Influence of whole-body irradiation with low-dose γ-rays on amino acid neurotransmitter levels in mice brain tissue |
| 投稿时间：2011-10-30 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.02.018 |
| 中文关键词: γ射线|小鼠|小剂量照射|神经递质 |
| 英文关键词:γ-rays|Mice|Low-dose irradiation|Neurotransmitters |
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| 中文摘要: |
| 目的 探讨小剂量γ射线全身照射后对小鼠中枢神经系统的影响。方法 将50只C57小鼠按随机表法分为健康对照组及0.5和1 Gy照射组。用60Co γ射线全身照射,吸收剂量为0.5和1 Gy,吸收剂量率为19.2 cGy/min,连续照射3 d,1次/d,照射后24和48 h取小鼠脑组织并匀浆,用高效液相色谱荧光法(HPLC)测定匀浆上清中氨基酸类神经递质(谷氨酸、天冬氨酸、γ-氨基丁酸及甘氨酸)含量。结果 与健康对照组相比,0.5 Gy γ射线照射后48 h以及1 Gy照射后24和48 h,小鼠脑内谷氨酸和天冬氨酸含量表达增高(t=-4.080、-3.935、-4.416、-3.630、-4.831和-4.656,P＜0.05);而0.5 Gy γ射线照射后24 h小鼠脑内谷氨酸、天冬氨酸含量无明显改变。各照射组小鼠脑内γ-氨基丁酸和甘氨酸含量与健康对照组比较,差异无统计学意义。结论 小剂量γ射线短期全身照射对小鼠中枢神经系统具有轻度的兴奋作用。 |
| 英文摘要: |
| Objective To investigate the effect of whole-body irradiation with low-dose γ-rays on the central nervous system of mice. Methods Fifty C57 mice were randomly divided into 3 groups and treated with 0, 0.5, 1 Gy whole-body irradiation, respectively. 24 or 48 h after irradiation, brain tissue of mice was resected and homogenated. The levels of amino acid neurotransmitter, including Glu, Asp, GABA and Gly in brain homogenate were measured by high performance liquid chromatography (HPLC). Results Compared to the brain tissue of untreated mice, the contents of Glu and Asp at 0.5 and 1 Gy (t=-4.080,-3.935,-4.416,-3.630,-4.831, -4.656,P＜0.05) in mice brain tissue significantly increased at 24 h at 1 Gy and 48 h. However, the contents of Glu and Asp had no obvious changes in mice brain tissue 24 h after 1 Gy of irradiation. The contents of GABA and Gly had no difference between irradiated groups and untreated control group. Conclusions Short-term whole-body irradiation with low-dose γ-rays induces slight stimulation effect on the central nervous system of mice. |
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