| 赵琳,王彦苏,任航,等.乙醇降低人乳腺癌MCF-7细胞放射敏感性的研究[J].中华放射医学与防护杂志,2012,32(2):162-165.ZHAO Lin,WANG Yan-su,REN Hang,et al.Ethanol decreases radiosensitivity of human breast cancer MCF-7 cells[J].Chin J Radiol Med Prot,2012,32(2):162-165 |
| 乙醇降低人乳腺癌MCF-7细胞放射敏感性的研究 |
| Ethanol decreases radiosensitivity of human breast cancer MCF-7 cells |
| 投稿时间：2011-11-15 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.02.014 |
| 中文关键词: 乙醇|乳腺癌|放射敏感性|细胞周期|细胞凋亡 |
| 英文关键词:Ethanol|Breast cancer|Radiosensitivity|Cell cycle|Apoptosis |
| 基金项目:国家自然科学基金(81071906;81172127);长江学者和创新团队发展计划资助项目(IRT0849);江苏省省属高校自然科学重大基础研究项目(SZ126933);江苏省高等学校优秀科技创新团队项目(SZ132901);江苏省高校优势学科建设工程资助项目(PAPD) |
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| 中文摘要: |
| 目的 研究乙醇对人乳腺癌MCF-7细胞放射敏感性的影响及相关机制。方法 将人乳腺癌MCF-7细胞分为4组,对照组(不做任何处理)、乙醇组(乙醇处理)、单纯照射组(6 Gy X射线处理)和联合组(乙醇与X射线联合作用)。克隆形成法检测50或100 mmol/L乙醇对MCF-7细胞放射敏感性的影响;流式细胞术检测细胞周期变化;Annexin V-FITC法检测细胞凋亡。结果 50和100 mmol/L乙醇处理MCF-7细胞50 h,对生长无明显影响(t=0.82和1.15,P>0.05);乙醇预处理2 h,可明显增加X射线照射后MCF-7细胞克隆形成的能力(t=4.15和10.28,P<0.05)。相比于单纯照射组,乙醇降低了X射线照射诱导的细胞G2/M期阻滞(t=7.18,P<0.05),以及sub-G1峰的比例(t=5.39,P<0.05)。而且,Annexin V-FITC检测结果示,乙醇联合X射线照射的细胞晚期和早期凋亡减少(t=4.86和7.59,P<0.05)。结论 乙醇可以增加MCF-7细胞的辐射抗性,其机制可能与降低辐射诱导的细胞G2/M期阻滞及早、晚期凋亡的发生相关。 |
| 英文摘要: |
| Objective To investigate the effect of ethanol on radiosensitivity of human breast cancer MCF-7 cells. Methods Human breast cancer MCF-7 cells were divided into four groups including control group, ethanol treatment group, X-ray exposed group, and ethanol combined with X-ray group. Clonogenic assay was used to determine cell survival. Flow cytometry was employed to analyze cell cycle progression. Annexin V-FITC kit was used to determine cell apoptosis induction. Results Ethanol(50 and 100 mmol/L,50 h)had no influence on MCF-7 cell growth(t=0.82,1.15,P>0.05). The radiosensitivity of MCF-7 cells was reduced when the cells were pretreated with 50 mmol/L ethanol (t=4.15,P<0.05) and 100 mmol/L ethanol(t=10.28,P<0.05)for 2 h. Compared with irradiation with X-ray alone, ethanol treatment decreased G2/M phase arrest(t=7.18,P<0.05) and sub-G1 population(an indicator of apoptosis induction)(t=5.39,P<0.05). A decrease of advanced and early apoptosis in the cells pretreated with ethanol was also confirmed by Annexin V-FITC apoptosis assay(t=4.86,7.59,P<0.05). Conclusions Ethanol causes radioresistance in human breast cancer MCF-7 cells, where the decreases of radiation-induced G2/M phase arrest and apoptosis may be involved. |
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