| 张兵华,费晋秀,郭永博,王红霞,宋晓峰,闻勤生,黄裕新.高功率脉冲微波辐照对大鼠胰腺及血清一氧化氮和内皮素的影响[J].中华放射医学与防护杂志,2012,32(1):52-55 |
| 高功率脉冲微波辐照对大鼠胰腺及血清一氧化氮和内皮素的影响 |
| Effect of high power pulse microwave on morphological changes of pancreas and nitric oxide and endothelin in blood serum in rats |
| 投稿时间：2010-12-01 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.012 |
| 中文关键词: 高功率脉冲微波 一氧化氮 内皮素 胰腺 |
| 英文关键词:High power pulse microwave Nitric oxide Endothelin Pancreas |
| 基金项目: |
|
| 摘要点击次数: 3343 |
| 全文下载次数: 1854 |
| 中文摘要: |
| 目的 观察不同剂量高功率脉冲微波辐照对SD大鼠胰腺形态学及血清一氧化氮、内皮素的影响。方法 80只SD大鼠随机分为4组(n=20),其中1组为对照组,其余3组为辐照组,各组再按取材时间分为14、24、48及72 h 4个亚组(n=5),辐照组分别接受104、105、4×105脉冲高功率脉冲微波辐照,辐照后14、24、48及72 h观察胰腺光镜、电镜下的形态学改变,分别以生物化学法、放射免疫法测定血清淀粉酶、一氧化氮(NO)及内皮素(ET)含量。结果 辐照组大鼠光镜下可见胰腺毛细血管扩张充血,电镜下104、105脉冲组可见胰腺细胞轻度损伤,表现为线粒体肿胀、粗面内质网扩张及核内染色质边集;4×105脉冲组损伤更加明显,各组均以24和48 h损伤最为严重。与对照组相比,在辐照后14和24 h,辐照组血清淀粉酶降低(F=12.58、11.73,P<0.05),而内皮素含量升高(F=4.50、4.49,P<0.05),同时在辐照后各时间点NO含量均增高(F=17.51、41.72、19.98、32.64, P<0.05),随辐照剂量的增加,NO升高越明显。结论 高功率脉冲微波可引起大鼠胰腺毛细血管扩张和胰腺细胞轻度损伤,损伤程度与辐射剂量有一定的关系,辐射剂量越大,损伤越严重,其机制可能与血清内皮素和NO升高有关。 |
| 英文摘要: |
| Objective To observe the morphological changes of rats' pancreas and nitric oxide(NO) and endothelin(ET) in the blood serum in rats after exposure to different pulses of high power pulse microwave (HPPMW). Methods SD-rats were irradiated with 104, 105 and 4×105 pulses of HPPMW, respectively. After gloss observation, the histopathological changes of pancreas were observed through biological microscope and electroscope. The changes of amylase, nitric oxide and endothelin in blood serum were detected by biochemical and radio-immunological methods. Results Compared with the blank control, no apparent abnormality could be observed in the pancreas of all groups. The dilatation of capillary could be observed in each experimental group by microscope. The ultrastructure changes of pancreas were most serious in 4×105 pulse group, especially at 24 and 48 h after irradiation. Compared with the control group, the levels of serum amylase were decreased (F=12.58,11.73, P<0.05), while ET were increased (F=4.50, 4.49, P<0.05) at 24 and 48 h after irradiation. The levels of NO in serum were increased (F=17.51, 41.72, 19.98,32.64, P<0.05) at each time-point. The level of NO went up with the increase of pulses. Conclusions HPPMW has damage effects on the pancreas in rats. The pulses with the pancreas can lead to severity of the damage. The mechanism of HPPMW may be involved in the enhancement of ET and NO in serum. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|