| 徐加英,吉俊敏,焦旸,伍理,樊赛军.血根碱对卵巢癌细胞生长及放射敏感性的影响[J].中华放射医学与防护杂志,2012,32(1):6-10,14 |
| 血根碱对卵巢癌细胞生长及放射敏感性的影响 |
| Effect of sanguinarine on the growth and radiosensitivity of human ovarian cancer cells |
| 投稿时间：2011-04-11 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.002 |
| 中文关键词: 血根碱 卵巢癌 细胞周期 凋亡 放射增敏性 |
| 英文关键词:Sanguinarine Ovarian cancer Cell cycle Apoptosis Radiosensitivity |
| 基金项目:教育部"长江学者和创新团队发展计划资助"项目(IRT0849);江苏省普通高校研究生科研创新计划(CX10B-0452);苏州大学优秀博士学位论文选题立项(233209749) |
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| 中文摘要: |
| 目的 探讨血根碱对卵巢癌SK-OV-3细胞的生长及放射敏感性的影响。方法 采用MTT法和克隆形成法检测血根碱处理后卵巢癌SK-OV-3细胞的生长;流式细胞仪分析方法观察血根碱对细胞周期分布和细胞凋亡的影响;Annexin V/PI法观察血根碱联合辐照对细胞凋亡的影响。结果 0~5 μmol/L血根碱处理24和48 h后,细胞生长的抑制与血根碱处理的剂量和时间成正相关(r=0.96和0.97),半数细胞生长抑制的浓度(IC50)分别为3.02和1.11 μmol/L。血根碱处理导致了包括细胞变圆、皱缩,漂浮细胞增多等细胞形态学的改变。Sub-G1凋亡峰随血根碱浓度上升而增高,并出现G0/G1期细胞周期阻滞。0.5 μmol/L血根碱预处理24 h后经6 Gy的X射线辐照,早期凋亡细胞从10.28%增加至43.28%(t=19.41, P<0.01),晚期凋亡细胞从20.26%增加至30.80%(t=8.78, P<0.01)。采用多靶单击模型拟合曲线发现血根碱+ X射线组与X射线单独照射组的放射增敏比(SERD0)达1.625,即低浓度的血根碱可以增加卵巢癌细胞的放射敏感性。结论 血根碱可以显著抑制卵巢癌SK-OV-3细胞的生长,诱导细胞凋亡和周期阻滞,而且在低剂量下,血根碱可以增加卵巢癌细胞的放射治疗敏感性。 |
| 英文摘要: |
| Objective To study the effect of sanguinarine on the growth and radiosensitivity of ovarian cancer SK-OV-3 cells. Methods Cell growth was determined by MTT and clonogenic assay. Cell cycle analysis was performed by flow cytometry assay. The cell apoptosis was analyzed by Annexin V/PI assay. Results Sanguinarine inhibited SK-OV-3 cell growth in a dose-and time-dependent fashion and its IC50 values were 3.02 and 1.11 μmol/L at 24 and 48 h, respectively. Sanguinarine also significantly triggered a sub-G1 peak, an indicator of apoptosis, and caused a G0/G1 arrest. Furthermore, the cell apoptosis induced by X-irradiation was significantly increased at 6 Gy when the cells were pre-treated with sanguinarine, in which the early apoptotic population increased from 10.28% to 43.28% (t=19.41, P<0.01) and the late apoptotic population increased from 20.26% to 30.80% (t=8.78, P<0.01). The multi-target click model was used to fit survival curves and the SER of sanguinarine treatment approached to 1.625 at the dose of D0. Conclusions Sanguinarine could inhibit SK-OV-3 cell growth by inducing apoptosis and cell cycle arrest and enhance cell radiosensitivity at low doses. |
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