| 任瑞平,何明远,董忱,谢月霞,邵春林.不同LET射线及其联合照射下淋巴母细胞微核率的剂量效应[J].中华放射医学与防护杂志,2012,32(1):1-5 |
| 不同LET射线及其联合照射下淋巴母细胞微核率的剂量效应 |
| Dose-response of micronuclei frequency in lymphoblast cells irradiated with different LET rays and combination of α-particles and γ-rays |
| 投稿时间：2011-08-27 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.001 |
| 中文关键词: α粒子 γ射线 联合照射 微核率 剂量-效应 |
| 英文关键词:α-particle γ-ray Mixed irradiation Micronucleus Dose-response |
| 基金项目:国家自然科学基金(31070758;11179002) |
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| 中文摘要: |
| 目的 探讨在α粒子、γ射线以及联合照射下淋巴母细胞微核率的剂量效应。方法 以HMy2.CIR淋巴母细胞为对象,分别以不同剂量的α粒子和γ射线进行照射,以及先以0.025~0.500 Gy的α粒子照射后即刻以不同剂量的γ射线进行照射,用胞质分裂阻断法检测淋巴细胞微核,建立不同照射条件下淋巴母细胞微核率的剂量-效应曲线。结果 对于γ射线照射,微核率剂量-效应符合线性平方模型Y=c+αD+βD2。对于α粒子照射,当照射剂量<0.250 Gy时,细胞微核率随剂量的增加呈线性增加,当其剂量进一步增加时,微核率剂量-效应曲线呈现下弓型,可以采用反映辐射旁效应的BaD模型Y=c+αD+σ[1-exp(-δD)]exp(-βD)进行很好地拟合。对于联合照射,当α粒子剂量较低时,微核率的剂量效应与γ射线照射时的相似;但当α粒子剂量较大时,微核率的剂量-效应更接近于α粒子照射。同时,0.2、0.5 Gy α粒子联合γ射线照射引起的微核率显著高于它们单独照射时的微核率之和(t=5.22~11.86,P<0.05)。结论 α粒子照射具有与γ射线不同的辐射损伤规律,辐射旁效应可能在其中发挥了重要作用,而联合照射则可以引起细胞损伤的协同增强。 |
| 英文摘要: |
| Objective To investigate the dose-response of micronuclei (MN) frequency in the lymphocytes irradiated with or without combination of α-particles and γ-rays. Methods Human lymphoblast cells HMy2.CIR were irradiated with 0-1 Gy of α-particles, 0-5 Gy of γ-rays, and 0.025-0.5 Gy of α-particles followed by different doses of γ-rays, respectively. The micronuclei(MN) in the irradiated cells were measured with the cytokinesis block technique, and the dose-responses of MN were established under different irradiation conditions. Results For γ-ray irradiation, the dose-response of MN was well-fit by the linear-quadratic model with an equation Y=c+αD+βD2.For α-particle irradiation, the MN induction increased linearly with the dose less than 0.250 Gy. But when the dose of α-particles increased continually, the dose-response curve bended and could be well fit with the BaD model Y=c+αD+σ[1-exp(-δD)]exp(-βD) where radiation-induced bystander effect (RIBE) was indicated. For the combined exposure, the dose-response of MN was similar to that of γ-irradiation when the dose of α-particles was lower than 0.1 Gy, but it was similar to that of α-irradiation when the dose of α-particles was higher. When the dose of α-particles was 0.2 and 0.5 Gy, MN induced by the mixed radiation were significantly higher than the sum of corresponding irradiation alone (t=5.22-11.86, P<0.05). Conclusions The radiation damage of α-particles differs from that of γ-rays, where RIBE may be involved. The combination irradiation of α-particles and γ-rays has a synergistic effect on radiation damage of lymphoblast cells. |
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