| 杨爱洁,何信佳,安永恒,王鑫.不同分割照射对胰腺生物学效应的影响[J].中华放射医学与防护杂志,2011,31(6):653-656 |
| 不同分割照射对胰腺生物学效应的影响 |
| Biological effects of different fractionated irradiation on pancreas after |
| 投稿时间：2011-02-05 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.06.008 |
| 中文关键词: 辐射效应 放射损伤 胰腺 |
| 英文关键词:Radiation effects Radiation injuries Pancreas |
| 基金项目:青岛市公共领域科技支撑计划项目(09-1-1-40-nsh) |
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| 中文摘要: |
| 目的 探讨不同分割模式X射线照射后大鼠胰腺的损伤程度。方法 将健康成年Wistar大鼠80只,随机分为常规分割组,12 Gy分6次照射,每天1次;单次大剂量组,一次性照射12 Gy;中等低分割组,12 Gy分2次照射,中间间隔4 d;对照组,给予假照;每组20只。于照射前及照射后4、7、14、21和42 d称重、测空腹血糖、淀粉酶,观察胰腺组织学改变。结果 各实验组血糖照射后4 d时降至最低,最低时达(3.1±0.1)mmol/L,(LSD-t=20.06~28.74,P<0.001),单次大剂量组较常规分割组、中等低分割组降低更明显(LSD-t=8.72~9.71,P<0.001),常规分割组、中等低分割组差异无统计学意义,14 d及以后接近正常;血淀粉酶照射后4和7 d时升高,最高时可达(84.5±6.4 )U/L(Dunnett's-t=23.10~46.10,P<0.001),7 d时单次大剂量组较常规分割组、中等低分割组升高更明显(Dunnett's-t=7.11,P<0.05),常规分割组与中等低分割组比较差异无统计学意义,14 d及以后恢复正常。光镜下,单次大剂量组4 d时个别腺泡变性坏死,14 d时腺管周围间质纤维增生,21 d时胰岛细胞间纤维增生,42 d时腺细胞增生。常规分割组、中等低分割组出现同样变化,仅程度较轻,出现较晚。结论 当分次剂量及间隔时间合适时,中等低分割照射损伤程度未超过常规照射,这对临床非常规放疗的开展有一定的参考价值。 |
| 英文摘要: |
| Objective To investigate the damages on pancreas after different fractionated irradiation in rats. Methods Eighy healthy adult Wistar rats were randomly divided into four groups with 20 rats in each group as conventional fractionated irradiation group with 2 Gy per fraction to a dose of 12 Gy,hypofractionated radiation group with one fraction of 12 Gy,middle-dose fractionated radiation group with 6 Gy per fraction to a dose of 12 Gy in the interval of 4 days and control group without radiation. Changes in weight, fasting blood glucose and amylase were measured and morphological changes were observed in different periods. Results In the experimental groups, the reduction was observed in fasting glucose at 4 d, reached a minimum of (3.1±0.1)mmol/L,(LSD-t=20.06-28.74, P<0.001) and the increase of amylase was found after 4th and 7th day, reached a maximum of (84.5±6.4) U/L(Dunnett's-t=23.10-46.10,P<0.001), both more obvious in hypofractionated radiation group than those of conventional fractionated radiation group and middle-dose fractionated radiation group (LSD-t=8.72-9.71, Dunnett's-t=7.11,P<0.05) ,however the levels in conventional fractionated radiation group was nearly to middle-dose fractionated radiation group (P>0.05) and became normal at 14 d. Under light microscope,the necrosis of acinarcells was observed in hypofractionated radiation group at 4th d, interstitial fibrogenesis were found at 14 d, the fibrogenesis were found in pancreatic island at 21 d, and the hyperplasia of acinarcells was observed at 42 d. The same changes were found in conventional fractionated radiation group and middle-dose fractionated radiation group, which were gently and lately than those of hypofractionated radiation group. Conclusions Radiation injury is not more serious after middle-dose fractionated radiotherapy than that after conventional fractionated irradiation, when the proper fractional dose and intervals are chosen. |
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