| 韩光,张慧,谢丛华,周云峰.GATA-3在放射性肺纤维化中的作用机制[J].中华放射医学与防护杂志,2011,31(6):631-635 |
| GATA-3在放射性肺纤维化中的作用机制 |
| Expression of GATA-3 in mice with radiation-induced lung fibrosis |
| 投稿时间：2011-03-26 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.06.003 |
| 中文关键词: 放射性肺纤维化 转录因子GATA-3 白细胞介素-13(IL-13) |
| 英文关键词:Radiation-induced lung fibrosis GATA-3 Interleukin-13 |
| 基金项目:国家自然科学基金(30770653) |
|
| 摘要点击次数: 3633 |
| 全文下载次数: 2218 |
| 中文摘要: |
| 目的 通过建立小鼠放射性肺纤维化模型,了解转录因子GATA-3及白细胞介素-13(IL-13)在放射性肺纤维化形成过程中的表达情况及其意义,为放射性肺纤维化的防治提供实验和理论依据。方法 成年雌性C57BL/6小鼠63只,随机分作2组:对照组21只, 不做任何处理;照射组42只,全肺单次照射12 Gy。于照射后1 h、1、2、4、8、16和24周,取照射组及相同鼠龄对照组小鼠肺组织和血清,分别用HE和Masson染色在光镜下观察组织病理改变和胶原纤维沉积;用碱水解法测定羟脯氨酸含量;用实时定量RT-PCR法检测GATA-3mRNA相对含量;用ELISA法测定血清中IL-13的含量;用Western blot法检测肺组织中GATA-3的蛋白表达变化情况。结果 光镜下,照射组小鼠肺组织较对照组组织学改变和胶原纤维沉积明显;测定羟脯氨酸含量显示,照射组小鼠肺组织中的羟脯氨酸含量较对照组明显增高(Z=3.142,P＜0.05);蛋白和mRNA检测结果显示,较之对照组,GATA-3和IL-13的表达在照射组中明显升高,特别是在肺组织尚未发生明显的纤维化组织学改变时(1~2周),照射组GATA-3表达明显升高(t=6.50,6.33,P＜0.01),IL-13受GATA-3调节,在照射后第16周表达最为明显(t=32.21,P＜0.01)。结论 转录因子GATA-3可以通过诱导Th2免疫细胞因子IL-13的表达,参与放射性肺纤维化的形成。 |
| 英文摘要: |
| Objective To observe the expression of GATA-3 and IL-13 in mice with radiation-induced lung fibrosis, and to study the function of GATA-3. Methods A total of 63 C57BL/6 female mice were randomly into 2 groups, including 21 mice in control group and 42 mice in irradiated group.The thoraces of mice in irradiated group were exposed with 12 Gy of X-rays. All the mice were sacrificed at 1 h, and 1, 2, 4, 8, 16 and 24 weeks post-irradiation. The lung issues were stained by using HE and Masson methods to determine the histological changes. The expression of IL-13 in serum, and the expression of hydroxyproline and the mRNA and protein of GATA-3 in lung tissue were assayed. Results Compared with control group, there was a significant histological and pathologic change in irradiated group. The content of hydroxyproline in irradiated group was significantly higher than that in control group(Z=3.14,P<0.05).The expressions of GATA-3 and IL-13 were found in mice post-irradiation. Without causing conspicuous fibrotic pathological changes, there was a significantly elevated expression of Th2-specific transcription factor GATA-3 mRNA at 1 and 2 weeks post-irradiation (t=6.50, 6.33, P＜0.01), while the expression of IL-13 reached the maximal value in serum at 16 weeks post-irradiation(t=32.21, P＜0.01). Conclusions GATA-3 might play a role in promoting radiation-induced pulmonary fibrosis by up-regulation of expression of Th2 cytokine IL-13. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|