| 冯晓青,胡志勇,刘华清,等.甲状旁腺素对辐射损伤小鼠造血功能的防护作用[J].中华放射医学与防护杂志,2011,31(5):542-544.FENG Xiao-qing,HU Zhi-yong,LIU Hua-qing,et al.Protective effects of parathyroid hormone on radiation-induced hematopoietic damage in mice[J].Chin J Radiol Med Prot,2011,31(5):542-544 |
| 甲状旁腺素对辐射损伤小鼠造血功能的防护作用 |
| Protective effects of parathyroid hormone on radiation-induced hematopoietic damage in mice |
| 投稿时间：2010-10-12 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.05.008 |
| 中文关键词: 甲状旁腺素 辐射损伤 造血功能 |
| 英文关键词:Parathyroid hormone Radiation damage Hematopoiesis |
| 基金项目:江苏省自然科学基金(BK2009120) |
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| 中文摘要: |
| 目的 探讨重组人甲状旁腺素(parathyroid hormone, PTH)对辐射所致小鼠造血功能损伤的防护作用。方法 雄性C57小鼠60只,经60Co γ射线照射后随机分为PTH治疗组和照射对照组。PTH治疗组每天给予PTH 80 μg ·kg-1 ·d-1 腹腔注射,对照组给予等量生理盐水。全自动血细胞分析仪检测小鼠外周血细胞数,手工计数骨髓有核细胞数,流式细胞仪分析骨髓中CD34+细胞数,集落细胞培养法观察骨髓粒系造血祖细胞数。结果 在照射后10、15和20 d,PTH治疗组小鼠的外周血红细胞计数显著高于照射对照组(t=6.48、3.66和4.98,P<0.05)。在照射后各时间点外周血白细胞计数显著高于照射对照组(t=6.32、9.19、11.18、7.44和4.42,P<0.05)。在照射后5和30 d血小板计数显著高于照射对照组(t=2.57和3.10,P<0.05)。在照射后各时间点PTH治疗组骨髓有核细胞数均显著高于照射对照组(t=5.80、3.72、17.89、4.90和16.49,P<0.05)。PTH显著增加了照射后小鼠的骨髓粒细胞集落形成单位数和CD34+细胞数(t=16.12、7.82和20.00,P<0.05)。结论 甲状旁腺素对辐射引起的造血损伤具有明显的保护作用。 |
| 英文摘要: |
| Objective To study the protective effects of parathyroid hormone (PTH) on radiation-induced hematopoietic damage in mice. Methods A total of sixty male C57 mice were irradiated by 60Co γ-rays to induce hematopoietic injuries, and then the mice were randomly divided into PTH and control group. The PTH-treated group was treated with PTH (80 μg ·kg-1 ·d-1) intraperitoneally everyday. The control group was given equivalent volume saline. Peripheral blood cell number, bone marrow mononuclear cell number, granulocyte-macrophage colony forming units(CFU-GM) and CD34 positive cells in bone marrow were detected. Results With the whole post-irradiation period, the WBC and bone marrow mononuclear cell numbers in PTH-treated mice were significantly higher than those in saline-treated mice(t=6.32, 9.19, 11.18, 7.44 and 4.42,P<0.05). The RBC numbers in PTH-treated mice were significantly higher than those in control mice at 10 d, 15 d and 20 d post-irradiation (t=6.48, 3.66 and 4.98, P<0.05). The PLT numbers in PTH-treated mice were significantly higher than those in control group at 5 and 30 d post-irradiation (t=2.57 and 3.10, P<0.05). PTH increased CD34 positive cell and CFU-GM numbers in bone marrow after irradiation(t=16.12, 7.82 and 20.00, P<0.05). Conclusions PTH could improve the hematopoietic recovery after irradiation. |
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