| 苏颖,何火聪,吴君心,等.鼻咽癌细胞放射敏感性的比较蛋白质组学研究[J].中华放射医学与防护杂志,2011,31(5):536-541.SU Ying,HE Huo-cong,WU Jun-xin,et al.Comparative proteomic study of nasopharyngeal carcinoma cell lines with different radiosensitivity[J].Chin J Radiol Med Prot,2011,31(5):536-541 |
| 鼻咽癌细胞放射敏感性的比较蛋白质组学研究 |
| Comparative proteomic study of nasopharyngeal carcinoma cell lines with different radiosensitivity |
| 投稿时间：2010-10-21 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.05.007 |
| 中文关键词: 鼻咽癌 放射敏感性 蛋白质组学 质谱 |
| 英文关键词:Nasopharyngeal carcinoma Radiosensitivity Proteomics Mass spectrometry |
| 基金项目:福建省自然科学基金(C0710004) |
|
| 摘要点击次数: 4148 |
| 全文下载次数: 2392 |
| 中文摘要: |
| 目的 探讨与鼻咽癌细胞放射敏感性相关的并可能预测鼻咽癌细胞放射敏感性的蛋白质。方法 以人鼻咽癌细胞株CNE-2诱导建立放射抗拒的鼻咽癌细胞株CNE-2(R743),通过克隆形成实验及流式细胞术检测以比较CNE-2和CNE-2(R743)细胞的放射敏感性及细胞周期的特征。提取细胞总蛋白质,进行双向凝胶电泳,Image Master 7.0图像分析软件分析图谱以识别差异蛋白质点,MALDI-TOF/TOF肽质量指纹图谱分析,蛋白质质谱数据库搜索鉴定差异蛋白。应用RT-PCR和Western blot验证2种细胞中相关差异蛋白的表达。 结果 得到7个差异表达较显著的蛋白质,与CNE-2相比较,CNE-2(R743)细胞中6个上调,1个下调。Western blot及 RT-PCR证实膜联蛋白A2、原肌球蛋白及糖调节蛋白78在CNE-2(R743)的表达均上调(t=24.22、24.20和29.19,P<0.05),与蛋白质组学结果一致。结论 不同放射敏感性鼻咽癌细胞存在差异表达的蛋白质,其中膜联蛋白A2、原肌球蛋白及糖调节蛋白78有可能成为预测鼻咽癌细胞放射敏感性的候选生物标志物。 |
| 英文摘要: |
| Objective To investigate the proteins which were associated with radiosensitivity of nasopharyngeal carcinoma(NPC) cells and could be used to predict the radiosensitivity. Methods A radioresistant subclone cell line CNE-2(R743) derived from NPC cell line CNE-2 was established. Radiosensitivity and cell cycle characteristics of CNE-2 and CNE-2(R743) were examined and compared by clonogenic survival assay and flow cytometry. The total proteins from the two cell lines were extracted and separated by two-dimensional gel electrophoresis, and the images were analyzed by Image Master 7.0 analysis software. Differentially expressed proteins in the two cell lines were identified through MALDI-TOF/TOF peptide mass fingerprint and searched in the protein sequence database. The protein expressions were confirmed by RT-PCR and Western blot. Results Totally seven differentially expressed proteins were identified, six of which were upregulated and one downregulated in the radioresistant CNE-2(R743), compared with those of CNE-2.Three out of the seven, Annexin A2, Tropomyosin 4 and GRP78 were upregulated in the CNE-2(R743), which were confirmed by Western blot and RT-PCR (t=24.22, 24.20, 29.19, P<0.05). Conclusions Differentially expressed proteins might be involved in different radiosensitivities of nasopharyngeal carcinoma cell lines, among which Annexin A2, Tropomyosin 4 and GRP78 could be the candidate biomarkers for predicting radiosensitivity of nasopharyngeal carcinoma cells. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|