中华放射医学与防护杂志

王立振,杨敏,徐宇平,等.³²P-磷酸铬-聚-L-乳酸粒子植入治疗裸鼠荷人胰腺癌移植瘤的研究[J].中华放射医学与防护杂志,2011,31(5):527-530.WANG Li-zhen,YANG Min,XU Yu-ping,et al.Experimental study of ³²P-CP-PLLA microparticle on human pancreatic carcinoma in nude mice[J].Chin J Radiol Med Prot,2011,31(5):527-530

³²P-磷酸铬-聚-L-乳酸粒子植入治疗裸鼠荷人胰腺癌移植瘤的研究

Experimental study of ³²P-CP-PLLA microparticle on human pancreatic carcinoma in nude mice

投稿时间：2010-08-27

DOI：10.3760/cma.j.issn.0254-5098.2011.05.005

中文关键词: 胰腺癌裸鼠 ³²P-磷酸铬-聚-L-乳酸粒子疗效不良反应

英文关键词:Pancreatic carcinoma Nude mice ³²P-CP-PLLA microparticle Therapeutic effect Toxic side-effect

基金项目:"863" 国家高技术研究发展计划(2007AA02Z471)

作者	单位
王立振	214063 无锡,江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室
杨敏	214063 无锡,江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室
徐宇平	214063 无锡,江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室
潘栋辉	214063 无锡,江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室
黄培林	东南大学核医学技术研究所
刘璐	东南大学核医学技术研究所
邵国强	南京医科大学附属南京第一医院临床核医学中心

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中文摘要:

目的探讨³²P-磷酸铬-聚-L-乳酸(³²P-CP-PLLA)粒子瘤体间质给药治疗BALB/c裸鼠荷人胰腺癌(BxPc-3)移植瘤的药效学及全身不良反应。方法 24只荷瘤裸鼠,随机分为4组:空白对照组、37.0、18.5、9.3 MBq组。经瘤体分别给予剂量为0、9.3、18.5和37.0 MBq ³²P-CP-PLLA粒子,动态观察各组相对肿瘤增殖率、体重变化,并计数WBC和PLT。注射后14 d取出瘤块,进行形态学观察及免疫组织化学分析。结果 ³²P-CP-PLLA粒子治疗组相对肿瘤增殖率均<40%。病理切片显示近粒子处肿瘤细胞变性坏死。与空白对照组比较,治疗组微血管密度(MVD)和Bcl-2表达强度均低于对照组,Bax表达强度高于对照组,治疗组Bcl-2/Bax比值明显下调, 0、9.3、18.5和37.0 MBq ³²P-CP-PLLA粒子组Bcl-2/Bax比值分别为3.83±0.43、2.19±0.57、1.10±0.32和0.47±0.13(t=2.36~2.77,P<0.05)。微血管密度分别为(31.2±2.3)、(23.8±1.5)、(14.8±0.8)和(11.0±1.2)个/视野。治疗组间的免疫组织化学分析指标差异有统计学意义(t=2.30~2.57,P<0.05)。结论 ³²P-CP-PLLA粒子瘤体间质给药是治疗胰腺癌安全、简便、有效的核素介入疗法。

英文摘要:

Objective To study the therapeutic and toxic effects of³²P-chromic phosphate-poly (L-lactic) acid (³²P-CP-PLLA) microparticle intratumoral administration into BALB/c nude mice bearing BxPc-3 human pancreatic carcinoma. Methods Twenty four nude mice bearing tumors were injected with 0, 9.3, 18.5 and 37.0 MBq ³²P-CP-PLLA microparticle,respectively. The relative tumor growth rates were observed every day, and white blood cells, platelets and body weight were measured. At 14 d after administration, the tumors were removed, histological examination and immunohistochemical analysis were performed. Results The relative tumor growth rates of each treatment group was lower than 40%. Histological examination showed the degenerative necrosis at the site nearby the mircoparticle. Immunohistochemical analysis showed that the Microvessel density (MVD) and the expression of Bcl-2 in treated group were lower than those in control group. In contrast, the expression of bax in treated group were higher than those in control group. The ratio of Bcl-2/Bax protein significantly decreased in the treatment group, which were 3.83±0.43, 0.47±0.13, 1.10±0.32, 2.19±0.57 for 0, 9.3, 18.5 and 37.0 MBq ³²P-CP-PLLA microparticle, respectively (t=2.36-2.77, P<0.05). MVD were 31.2±2.3, 23.8±1.5, 14.8±0.8, 11.0±1.2, respectively. Dose dependence was observed in both HE and IHC staining after 14 d treatment (t=2.30-2.57,P<0.05). Conclusions Intratumoral injection of ³²P-CP-PLLA microparticle might be a safe, easy and effective radionuclide interventional therapy for pancreatic carcinoma.

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