| 郑邈,郑凯,孙汉英,苏文霞,刘文励.成骨细胞促进急性放射损伤小鼠骨髓造血系统及血管恢复的研究[J].中华放射医学与防护杂志,2011,31(4):441-444 |
| 成骨细胞促进急性放射损伤小鼠骨髓造血系统及血管恢复的研究 |
| Effects of osteoblasts on recovery of hematopoiesis and angiogenesis in acute irradiation injured mice |
| 投稿时间：2010-07-28 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.04.016 |
| 中文关键词: 成骨细胞 放射损伤 造血 血管 |
| 英文关键词:Osteoblasts Irradiation injury Hematopoiesis Angiogenesis |
| 基金项目:高等学校博士学科点专项科研基金(200804871044) |
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| 中文摘要: |
| 目的 探讨成骨细胞对放射损伤小鼠骨髓造血系统及血管恢复的影响。方法 取18只雄性BALB/c小鼠股骨制备成骨细胞,其余42只小鼠随机分成健康对照组、成骨细胞组和生理盐水组3组。健康对照组不做任何处理;成骨细胞组和生理盐水组小鼠于6.0 Gy 60Co γ射线一次性全身均匀照射后,分别经尾静脉输入成骨细胞(2×106个/只)和等体积的生理盐水。于照射后第7、14和21天计数小鼠外周血细胞,骨髓单个核细胞并作骨髓组织学观察。采用流式细胞仪检测骨髓单个核细胞中CD34+细胞百分比,采用免疫组织化学方法检测小鼠骨髓微血管密度。结果 照射后第7、14和21天成骨细胞组小鼠外周血细胞和骨髓单个核细胞计数, 骨髓单个核细胞中CD34+细胞百分比,骨髓组织造血面积及骨髓微血管密度均明显高于生理盐水组(t=2.46~64.51,P<0.05)。结论 成骨细胞能促进放射损伤小鼠骨髓造血系统及血管的恢复。 |
| 英文摘要: |
| Objective To explore the effects of osteoblasts on the recovery of hematopoiesis and angiogenesis in acute irradiation injury mice. Methods The femurs of 18 male BALB/c mice were used to prepare the bone marrow osteoblasts, and the rest mice were divided into 3 groups as normal group, saline group and osteoblast group. The mice in normal group received no treatment, and the other two groups were received 6.0 Gy 60Co γ-ray irradiation. After irradiation each mouse of osteoblast group was administered with 2×106 osteoblasts through tail vein injection, and equal volume saline was given to each mouse of saline group by the same way. The following factors were measured at 7, 14, 21 d after irradiation, they were the counts of peripheral blood cells and bone marrow mononuclear cells (BMMNC), the percentage of CD34+ cells in BMMNC, the histology changes and micro vascular density (MVD) of bone marrow tissue. Results The counts of peripheral blood cells, BMMNC and hematopoietic tissue area in osteoblast group were higher than those in saline group. The percentage of CD34+ cells in BMMNC and the MVD of bone marrow in osteoblast group were also higher than those in saline group at 7, 14, 21 d after irradiation(t=2.46-64.51,P<0.05). Conclusions Osteoblasts could significantly promote the recovery of hematopoiesis and angiogenesis in mice after acute irradiation injury. |
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