| 王翠兰,江其生,邹跃,李峰生,李伟,宋秀军,何蕊,王璐.X射线对人肺癌A549细胞CCR7表达影响的研究[J].中华放射医学与防护杂志,2011,31(3):299-302 |
| X射线对人肺癌A549细胞CCR7表达影响的研究 |
| Effects of X-rays on CC-chemokine receptor 7 expression in human lung cancer A549 cells |
| 投稿时间：2010-12-02 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.013 |
| 中文关键词: 肺癌 CC-趋化因子受体7 X射线 |
| 英文关键词:Lung cancer CC-chemokine receptor 7 (CCR7) X-rays |
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| 中文摘要: |
| 目的 研究不同剂量X射线照射及照射后不同时间点对人肺癌A549细胞CC-趋化因子受体7(CCR7)表达的影响。方法 体外培养A549细胞,实验组采用直线加速器X射线一次性照射,细胞吸收剂量分别为2、4、6和8 Gy(源皮距100 cm;剂量率442.89 cGy/min),照射后4、12、24、48和72 h分别采用实时荧光定量PCR技术及Western blot方法分别进行CCR7 mRNA及蛋白质表达水平检测;对照组A549细胞除不接受X射线照射外,余处理同实验组。结果 A549细胞经2、4、6和8 Gy的X射线照射后, CCR7 mRNA及蛋白质在照射4 h后开始表达升高,达到高峰后相继出现下降;72 h后6和8 Gy组mRNA表达量仍高于对照组水平(t=6.75~7.26, P <0.01),2和6 Gy组蛋白质表达量高于对照组(t=11.13~14.17, P <0.01),而4和8 Gy组蛋白质表达量在48和72 h已降至对照组水平。结论 2、4、6和8 Gy的X射线照射A549细胞后,A549细胞CCR7 mRNA及蛋白质的表达量明显增加,可能与一定剂量X射线辐射促进A549细胞增殖和转移有关。 |
| 英文摘要: |
| Objective To study the effects of X-ray radiation on CC-chemokine receptor 7 (CCR7) expression in human non-small cell lung cancer (NSCLC) cells. Methods Human adenocarcinoma cells of the line A549 were cultured and irradiated by X-ray at the absorbed doses of 2, 4, 6, and 8 Gy respectively by linear accelerator (with the source skin distance of 100 cm and dose rate of 442.89 cGy/min). The relative levels of CCR7 mRNA and protein expression in the A549 cells were respectively detected by real time-PCR and Western blotting 4, 12, 24, 48, and 72 h after radiation. Untreated A549 cells were used as control group. Results The expression levels of CCR7 mRNA and protein in the A549 cells began to increase since 4 h after radiation and then decreased gradually after they reached the peak. The CCR7 mRNA expression levels 72 h after radiation of the 6 and 8 Gy groups were still significantly higher than those of the control group (t=6.75-7.26,both P <0.01), and the CCR7 protein expression levels of the 2 and 6 Gy group were still significantly higher than those of the control group(t=11.13-14.17,both P <0.01). Then the CCR7 protein expression levels of the 4 and 8 Gy groups decreased to the control group level 48 and 72 h after radiation respectively. Conclusions The CCR7 mRNA and protein expression levels in the NSCLC cells increase after X-ray irradiation, which may be correlated with the promotion of proliferation and metastasis of NSCLC cells by X-ray irradiation at a certain dose. |
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