| 艾昊,白海,王存邦,欧剑锋,赵强,韩夏,胡晓燕,陈哲.低剂量X射线照射对脐带间充质干细胞增殖及成脂成骨分化的影响[J].中华放射医学与防护杂志,2011,31(3):290-293 |
| 低剂量X射线照射对脐带间充质干细胞增殖及成脂成骨分化的影响 |
| Effects of low dose X-ray irradiation on proliferation and differentiation of mesenchymal stem cells derived from human umbilical cord into adipocytes and osteoblasts |
| 投稿时间：2010-08-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.011 |
| 中文关键词: 低剂量 X射线 间充质干细胞 增殖 成脂细胞 成骨细胞 成骨细胞核结合因子α1 |
| 英文关键词:Low dose irradiation Human umbilical cord mesenchymal stem cells Proliferation Adipocytes Osteoblasts Core binding factor-α1 |
| 基金项目: |
|
| 摘要点击次数: 3088 |
| 全文下载次数: 2245 |
| 中文摘要: |
| 目的 探讨低剂量照射对人脐带间充质干细胞(hucMSCs)增殖及成脂、成骨分化的影响。 方法 采用组织块贴壁法从人脐带沃顿胶样组织中分离出hucMSCs,并采用流式细胞术检测表面特异性标记。随机分为未予照射的对照组,以及50、100和200 mGy照射组。采用MTT法检测hucMSC在不同照射剂量和不同时间(1~7 d)的增殖情况。取第3代细胞,随机分为照射组(给予200 mGy照射)和对照组(不予照射),在体外诱导其向脂肪、成骨细胞分化,并通过油红O染色、检测碱性磷酸酶(ALP)活性,用RT-PCR检测成骨细胞核结合因子α1 的mRNA表达,比较照射组与对照组细胞成脂、成骨分化的不同。 结果 9~12 d开始有成纤维细胞从组织块游出贴壁,2周左右达到80%。低剂量照射后2、 3、 4、 5和6 d,不同照射剂量组的细胞增殖均高于对照组(F =159.17、448.81、265.15、183.93、181.83,均 P ＜0.01),其中以100 mGy组促进细胞增殖作用最明显。照射组成脂诱导2 d后细胞内即有脂滴出现,且第21天成脂率明显高于对照组(t=28.25, P ＜0.01)。成骨诱导后,照射组ALP活性明显高于对照组(t=16.87, P ＜0.01),且成骨细胞核结合因子α1 的mRNA水平明显增高(t=14.16, P ＜0.01)。 结论 低剂量照射可以促进hucMSC增殖,并加速其向成脂、成骨细胞分化。 |
| 英文摘要: |
| Objective To observe the effects of low dose irradiation (LDR) on proliferation,adipogenesis and osteogenic potential of human umbilical cord mesenchymal stem cells (hucMSCs). Methods hucMSCs were isolated from Wharton's jelly tissue of human umbilical cord by modified tissue-piece inoculation, and flow cytometry was used to detect the expression of specific marker in the hucMSCs. The hucMSCs were randomly divided into two groups: irradiation group undergoing irradiation with the doses 50, 100, or 200 mGy respectively, and control group without irradiation. MTT method was applied to evaluate the proliferation of the hucMSCs at different time points with various doses irradiation. The third passage hucMSCs were randomly divided into two groups: irradiation group undergoing low dose irradiation of 200 mGy, and control group without irradiation, and then underwent induction by adipocytic and oesteocytic differentiation induction fluids respectively so as to differentiate into adipocytes and osteoblasts. Oil red O staining was used to detect the activity of alkaline phophatase (ALP), and RT-PCR was used to detect the mRNA expression of core binding factor alpha 1 in human osteoblast. Results After 9-12 days, fibroblasts began to swim out of the tissue piece with a confluence rate of 80% 2 weeks later. Within 7 days the absorption values of the hucMSCs undergoing different irradiation doses 2, 3, 4, 5, and 6 days later were all significantly higher than those of the control group(F =159.17, 448.81, 265.15, 183.93, and 181.83,all P ＜0.01), with the proliferation rates of the 100 mGy subgroup being the highest. After being induced liquid, vacuoles were observed in the irradiated group 2 days later. 21 days later, the adipogenic rates of irradiated group was significantly higher than that of the control group(t=28.25, P ＜0.01). The ALP activity increased in the irradiated group compared with control group(t=16.87, P ＜0.01).The expression level of Cbf-α1 mRNA was up-regulated obviously(t=14.16, P ＜0.01). Conclusions LDR promotes the proliferation of hucMSCs, and accelerates the hucMSCs’ differentiation into adipocytes and osteoblasts. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|