| 潘彬,曾令宇,程海,宋国梁,贾路,闫志凌,陈翀,徐开林.X射线和γ射线预处理对小鼠异基因骨髓移植后造血免疫重建的影响[J].中华放射医学与防护杂志,2011,31(3):260-263 |
| X射线和γ射线预处理对小鼠异基因骨髓移植后造血免疫重建的影响 |
| Effects of X-rays and γ-rays on reconstitution of hematopoiesis and immunity after allogeneic bone marrow transplantation |
| 投稿时间：2010-06-14 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.004 |
| 中文关键词: X射线 γ射线 同种异基因骨髓移植 免疫重建 |
| 英文关键词:X-rays γ-rays Allogeneic hematopoietic stem cell transplantation Immune reconstitution |
| 基金项目:国家自然科学基金(30971281) |
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| 中文摘要: |
| 目的 研究X射线和γ射线两种预处理方式造成的损伤程度的差别,以及对造血、免疫重建的影响,确定适用于异基因造血干细胞移植的预处理照射方式。方法 对受鼠分别使用直线加速器X射线或60Co γ射线进行致死剂量(总剂量为7.0 Gy)全身照射后,给予相同数量供鼠骨髓细胞移植。观察受鼠移植后的生存时间、重要脏器(肝、小肠和肺)病理变化、嵌合率(H-2Kb+细胞比例)和造血免疫重建状况。结果 移植后早期,γ射线移植组生存率高于X射线移植组,小肠和肺损伤程度亦较轻。嵌合率γ射线移植组5和10 d均高于X射线移植组(t=15.263、3.256, P <0.05)。γ射线移植组10和20 d外周血白细胞计数和淋巴细胞植入率均高于X射线移植组(t=3.624、6.695, P <0.05)。结论 与X射线相比,γ射线照射产生的预处理损伤较轻,可获得较好的造血、免疫重建效果,可提高移植模型的质量和实验的均一性。 |
| 英文摘要: |
| Objective To determine the conditioning regimen suitable for mice allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods Twelve BALB/c mice were randomly divided into 2 equal groups to undergo X-ray irradiation by linear accelerator at the dose of 7.0 Gy (pure X-ray group) or 60Co source irradiation at the dose of 7.0 Gy (pure γ-ray group). Thirty mice were randomly divided into 2 equal groups to undergo X-ray irradiation and then infusion of bone marrow from donor mice via caudal vein (X-ray+transplantation group) or γ-ray and then infusion of bone marrow via caudal vein (γ-ray+transplantation group). 3, 5, 7, 10, 15, 20, and 30 d later peripheral blood samples were collected to calculate the number of white blood cells (WBCs) and detect the chimeric rates of lymphocytes by flow cytometry. 5, 10, and 20 d after irradiation 15 mice were killed with their lung, liver, small intestine, spleen, and femurs taken out to undergo pathological examination. Results The survival rates during the period 5-15 days of the γ-ray+transplantation group were all significantly higher than those of the X-ray+transplantation group. The pathological changes of organs of the X-ray+transplantation group were all more severe than those of the γ-ray+transplantation group. Since the fifth day after transplantation cells originating from the donor began to appear in the peripheral blood. The chimeric rate of the γ-ray+transplantation group 10 days after transplantation was (95.53±2.57) %. The chimeric rates 5, 10, and 20 days after transplantation of the γ-ray+transplantation group were all significantly higher than those of the X-ray+transplantation group (t=15.263,3.256, P <0.05). The WBC count of both irradiation groups decreased to the lowest level 5 d later and began to increase 10 days after transplantation and the WBC counts of the γ-ray+transplantation group 10 and 20 days after transplantation were both significantly higher than those of the X-ray+transplantation group (t=3.624, 6.695, P <0.05). The chimeric rats of the peripheral lymphocytes 10 and 20 days after transplantation of the γ-ray+transplantation group were both significantly higher than those of the X-ray+transplantation group (t=12.317, 8.295, P <0.05).The homogeneity rate of transplantation of the γ-ray+transplantation group was better than that of the X- ray+transplantation group. Conclusions As a conditioning regimen in allogeneic hematopoietic stem cell transplantation γ-ray irradiation causes milder injury and accelerated reconstitution of hematopoiesis and immunity, in comparison with X-ray irradiation. |
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