中华放射医学与防护杂志

王璐,邹跃,江其生,等.X射线对人肺腺癌A549细胞Pokemon基因表达的影响[J].中华放射医学与防护杂志,2011,31(2):177-179.WANG Lu,ZOU Yue,JIANG Qi-sheng,et al.Effects of X-ray irradiation on expression of Pokemon gene in A549 cells of human lung adenocarcinoma[J].Chin J Radiol Med Prot,2011,31(2):177-179

X射线对人肺腺癌A549细胞Pokemon基因表达的影响

Effects of X-ray irradiation on expression of Pokemon gene in A549 cells of human lung adenocarcinoma

投稿时间：2010-10-12

DOI：10.3760/cma.j.issn.0254-5098.2011.02.015

中文关键词: 肺癌 Pokemon基因 A549细胞 X射线照射

英文关键词:Lung cancer Pokemon gene A549 cells X-rays Radiation

基金项目:全军"十一五"卫生科研规划资助项目(06MA035);国家自然科学基金(30271479)

作者	单位
王璐	215123 苏州大学放射医学与公共卫生学院
邹跃	解放军第二炮兵总医院
江其生	解放军第二炮兵总医院
李伟	解放军第二炮兵总医院
宋秀军	解放军第二炮兵总医院
周湘艳	中国疾病预防控制中心辐射防护与核安全医学所(周湘艳)
王翠兰	215123 苏州大学放射医学与公共卫生学院

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中文摘要:

目的研究不同剂量X射线照射及照射后不同时间点对人肺腺癌A549细胞Pokemon基因表达的影响。方法用吸收剂量分别为2、4、6和8 Gy 的X射线照射体外培养的人肺腺癌A549细胞, 2、4、8、12、24、48和72 h后,用实时定量PCR技术检测其中的Pokemon mRNA表达水平,以未照射组为对照。结果在2、4、6、8 Gy X射线照射后的早期(除2 Gy照射后的2和4 h外)Pokemon mRNA的表达降低,但在晚期(48 h以后)呈升高趋势,在大部分时间点实验组与对照组的差异有统计学意义(t=3.40~154.76,P<0.05)。结论较大剂量的X射线在早期可下调A549细胞Pokemon基因mRNA的表达,诱导肿瘤细胞凋亡;但在晚期又可诱导A549细胞高表达Pokemon mRNA,这可能与辐射所致A549细胞的DNA损伤修复和细胞周期调控有关。

英文摘要:

Objective To study the dose-and time-effects of X-ray irradiation on the expression of Pokemon gene in A549 cells of human lung adenocarcinoma. Methods A549 cells were cultured in vitro and exposed to X-rays with the doses of 2, 4, 6 and 8 Gy, respectively. Untreated A549 cells were used as control group. The relative levels of Pokemon mRNA expression in the cells were detected by using quantitative real-time PCR at 2, 4, 8, 12, 24, 48 and 72 h after irradiation. Results The Pokemon mRNA expression levels decreased in the early period after irradiation (except 2 and 4 h after irradiation in 2 Gy group) and then increased in the later stage (48 h after irradiation) with significant statistical differences at the most time points in comparison with the control group(t=3.40-154.76, P<0.05).Conclusions Higher doses of X-rays may degrade the expression of Pokemon mRNA in the human A549 cells and induce apoptosis in the early period, but also may upgrade its expression in the later period, which might be correlated with the cell cycle regulation and DNA damage repair in the A549 cells.

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