中华放射医学与防护杂志

郭亚,朱小东,曲颂,等.鼻咽癌放射抗拒相关的信号通路[J].中华放射医学与防护杂志,2011,31(2):167-171.GUO Ya,ZHU Xiao-dong,QU Song,et al.Radioresistance-related signaling pathways in nasopharyngeal carcinoma cells[J].Chin J Radiol Med Prot,2011,31(2):167-171

鼻咽癌放射抗拒相关的信号通路

Radioresistance-related signaling pathways in nasopharyngeal carcinoma cells

投稿时间：2010-04-23

DOI：10.3760/cma.j.issn.0254-5098.2011.02.013

中文关键词: 鼻咽癌信号通路放射抗拒基因芯片

英文关键词:Nasopharyngeal carcinoma Signaling pathway Radioresistance Gene chip

基金项目:国家自然科学基金(30860329);广西壮族自治区自然科学基金(桂科自0832229);广西2010年研究生教育创新计划(2010105981002M214);广西大型仪器协作共用网测试补助项目(851-2009-055)

作者	单位
郭亚	530021 南宁,广西医科大学附属肿瘤医院广西壮族自治区肿瘤防治研究所放疗科
朱小东	530021 南宁,广西医科大学附属肿瘤医院广西壮族自治区肿瘤防治研究所放疗科
曲颂	530021 南宁,广西医科大学附属肿瘤医院广西壮族自治区肿瘤防治研究所放疗科
苏芳	530021 南宁,广西医科大学附属肿瘤医院广西壮族自治区肿瘤防治研究所放疗科
王琪	530021 南宁,广西医科大学附属肿瘤医院第六实验室
张玮	530021 南宁,广西医科大学附属肿瘤医院第六实验室

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中文摘要:

目的研究具有不同放射抗拒性的人鼻咽癌细胞株CNE-2R与CNE-2的基因表达谱差异,筛选与鼻咽癌放射抗拒相关的基因信号通路。方法验证已构建的鼻咽癌放射抗拒细胞株CNE-2R的放射抗拒性,选用Human-6v3.0全基因组表达谱微珠芯片,筛选这2种细胞的差异表达基因。对差异基因进行生物信息学分析,寻找与鼻咽癌放射抗拒相关的信号通路。结果 CNE-2R与CNE-2相比,2次实验均出现的差异基因374个。差异基因的Kegg通路和Biocarta通路分析发现,Toll样受体信号通路和IL-1R介导的信号转导通路分别对鼻咽癌放射抗拒具有重要生物学意义。通路上的13个基因JUN、MYD88、CCL5、CXCL10、STAT1、LY96、FOS、CCL3、IL-6、IL-8、IL-1α、IL-1β、IRAK2表达差异具有统计学意义(t=13.47~66.57,P<0.05)。结论 Toll样信号受体通路和IL-1R介导的信号转导通路激活可能与鼻咽癌放射抗拒相关。

英文摘要:

Objective To study the difference of gene expression profile between the radioresistant human nasopharyngeal carcinoma cell line CNE-2R and CNE-2, and to screen the signaling pathway associated with radioresistance of nasopharyngeal carcinoma. Methods The radioresistant nasopharyngeal carcinoma cell line CNE-2R was constructed from the original cell line CNE-2. CNE-2R and CNE-2 cells were cultured and administered with ⁶⁰Co γ-ray irradiation at the dose of 400 cGy for 15 times. Human-6v 3.0 whole genome expression profile was used to screen the differentially expressed genes. Bioinformatic analysis was used to identify the pathways related to radioresistance. Results The number of the differentially expressed genes that were found in these 2 experiments was 374. The Kegg pathway and Biocarta pathway analysis of the differentially expressed genes showed the biological importance of Toll-like receptor signaling pathway and IL-1R-mediated signal transduction pathway to the radioresistance of the CNE-2R cells and the significant differences of 13 genes in these 2 pathways, including JUN, MYD88, CCL5, CXCL10, STAT1, LY96, FOS, CCL3, IL-6, IL-8, IL-1α, IL-1β, and IRAK2 (t=13.47-66.57,P < 0.05). Conclusions Toll-like receptor signaling pathway and IL-1R-mediated signal transduction pathway might be related to the occurrence of radioresistance.

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