| 倪冠英,武宁,郭海卓,等.辐射对Jurkat细胞P21蛋白及ICR小鼠胸腺细胞p21基因表达的影响[J].中华放射医学与防护杂志,2011,31(2):134-137.NI Guan-ying,WU Ning,GUO Hai-zhuo,et al.Effects of ionizing radiation on expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice[J].Chin J Radiol Med Prot,2011,31(2):134-137 |
| 辐射对Jurkat细胞P21蛋白及ICR小鼠胸腺细胞p21基因表达的影响 |
| Effects of ionizing radiation on expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice |
| 投稿时间：2010-12-01 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.02.005 |
| 中文关键词: 电离辐射 Jurkat细胞 P21蛋白 p21基因 胸腺细胞 脾细胞 |
| 英文关键词:Ionizing radiation Jurkat cell line P21 protein p21 gene Thymocyte Splenocyte |
| 基金项目:卫生行业科研专项(200802018) |
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| 中文摘要: |
| 目的 探讨电离辐射对Jurkat细胞P21蛋白和ICR小鼠胸腺细胞p21基因表达的影响。方法 采用流式细胞术(FCM),检测0、0.5、1.0、2.0、4.0及6.0 Gy X射线照射后Jurkat细胞中P21蛋白表达的变化。采用实时定量PCR技术,分别检测0、0.5、1.0、2.0、4.0及6.0 Gy X射线照射后4和24 h小鼠胸腺及脾细胞中p21基因表达的变化。结果 不同剂量 X射线照射后12和24 h,Jurkat细胞中P21蛋白表达在0.5~4.0 Gy范围内均随剂量的增大而升高( t =-24.23~-3.96, P <0.05), 6 Gy时均出现表达下降( t =-11.19、-14.50, P <0.05);与假照射组相比,在0~6.0 Gy照射后4 和24 h,小鼠胸腺及脾细胞中p21基因的相对表达量均随剂量增大逐渐增加( t =-29.96~8.80, P <0.05);并于6.0 Gy时达最高( t =-11.84~-3.42, P <0.05),仅胸腺细胞1 Gy照射后4 h除外( t =-3.42, P >0.05)。结论 X射线能诱导P21蛋白及基因表达增加,并在一定剂量范围内存在良好的剂量-效应关系。 |
| 英文摘要: |
| Objective To investigate the effects of ionizing radiation on the expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice. Methods Flow cytometry (FCM) was used to analyze the expression of P21 protein in Jurkat cells at 12 and 24 h after irradiation to 0, 0.5, 1.0, 2.0, 4.0, and 6.0 Gy. Real-time PCR was used to detect the expression of p21 gene in thymocytes and splenocytes of mice at 4 and 24 h after irradiation to 0, 0.5, 1.0, 2.0, 4.0, and 6.0 Gy. Multi-staining was used to analyze the micronucleus rates of Rct in bone marrow. Results The expressions of P21 protein were increased in a dose-dependent manner during 0.5-4.0 Gy( t =-24.23--3.96, P <0.05), but decreased at 6.0 Gy at 12 and 24 h post-irradiation( t =-11.19,-14.50, P <0.05). The expressions of p21 gene in both thymocytes and splenocytes of mice were increased in dose-dependent manner in the range of 0-6.0 Gy(including 6.0 Gy)( t =-29.96-8.80, P <0.05), and reached to the peak at 6.0 Gy at 4 and 24 h post-irradiation( t =-11.84--3.42, P <0.05), except thymocytes at 4 h and 1.0 Gy post-irradiation( t =-3.42, P >0.05). Conclusions The expressions of P21 protein and p21 gene could be increased by X-ray irradiation, which shows good dose-dependent manners in certain range of dose. |
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