| 刘建香,阮健磊,田梅,等.不同剂量60Co γ 射线部分照射人离体血对染色体畸变形成的影响[J].中华放射医学与防护杂志,2011,31(1):38-40.LIU Jian-xiang,RUAN Jian-lei,TIAN Mei,et al.Effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro [J].Chin J Radiol Med Prot,2011,31(1):38-40 |
| 不同剂量60Co γ 射线部分照射人离体血对染色体畸变形成的影响 |
| Effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro |
| 投稿时间：2010-03-30 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.010 |
| 中文关键词: 部分照射 人外周血 染色体畸变 |
| 英文关键词:Partial irradiation Human peripheral blood Chromosome aberration |
| 基金项目:卫生行业科研专项(200802018) |
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| 中文摘要: |
| 目的 分析不同剂量60Co γ 射线部分照射人离体血对淋巴细胞染色体畸变形成的影响。方法 用0~8 Gy (剂量率为0.35 Gy/min)60Co γ 射线在37 ℃条件下照射3份离体健康人外周血标本,以0.5 ∶1的比例与同一供血者的未受照血混合,120 min后进行培养、制片,显微镜下分析染色体畸变(双着丝粒+着丝粒环)的变化,借此进行剂量估算。结果 各组的双着丝粒体+着丝粒环和总畸变,以及断片和单体断裂均随着剂量的增加而增加。用双着丝粒+着丝粒环进行的剂量估算,0.5~2 Gy组大于照射剂量的1/3,4~8 Gy组均接近照射剂量的1/3。结论 染色体畸变可以作为估算非均匀照射的生物学指标之一。 |
| 英文摘要: |
| Objective To investigate the effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro. Methods The samples of heparinized peripheral whole blood from 3 healthy persons were exposed to 60Co γ-rays at the doses between 0 and 8 Gy with the dose rate of 0.35 Gy/min at the temperature of 37 ℃, and then mixed with the unirradiated blood samples of the same donors by the ratio of 0.5 ∶1. 120 minutes later the samples of mixed blood underwent routine culture. Microscopy was used to observe the chromosome aberration double (centromere+centromere) and the biological dose was estimated thereby. Results The amounts of double centromere+centromere were increased along with the dose of irradiation in all groups. The estimated biological dose was higher than the 1/3 of the irradiation dose when the dose was between 0.5 to 2 Gy, and was close to the 1/3 of the irradiation dose when the dose was between 4 to 8 Gy. Conclusion Chromosome aberration can be used as a biomarker in estimation of uneven irradiation. |
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