| 陈志明,赵银龙,潘兴喜,姜新,曲雅勤.氯氧喹对Lewis肺癌细胞及荷瘤小鼠的辐射增敏作用[J].中华放射医学与防护杂志,2011,31(1):33-37 |
| 氯氧喹对Lewis肺癌细胞及荷瘤小鼠的辐射增敏作用 |
| Radiation sensitizing effect of chloroxoquinoline on Lewis lung cancer cells and xenograft tumors in mice |
| 投稿时间：2010-07-30 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.009 |
| 中文关键词: 氯氧喹 Lewis细胞 辐射增敏 MTT比色法 |
| 英文关键词:Chloroxoquinoline Lewis cell Radiosensitization MTT assay |
| 基金项目:吉林省科技厅基金(200705221) |
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| 中文摘要: |
| 目的 研究氯氧喹联合X射线对Lewis肺癌细胞及荷瘤小鼠的辐射增敏作用。方法 细胞及动物实验均设置为对照组、给药组、照射组及给药+照射组(联合组)4个组别。采用体外细胞培养MTT检测及荷瘤动物模型观察方法,分别予以不同处理因素后,细胞实验用MTT法检测各组别细胞吸光度(A)值;小鼠皮下接种Lewis肺癌细胞,予以相关处理因素后,观察记录肿瘤生长情况并测量其长、宽径从而计算出肿瘤抑制率及肿瘤生长延迟。照射结束后眼静脉采血,检测各组小鼠血液标本中细胞成分数量的变化情况。结果 MTT实验结果显示,细胞照射24 h后,给药+照射组SF值较单纯照射组有一定的降低,但差异无统计学意义(P>0.05),而照射48 h后,在10、20、40和80 mg/L浓度下,两组间差异有统计学意义(t=3.22、4.23、4.46和4.58,P＜0.05)。动物实验结果显示,给药+照射组的肿瘤生长曲线明显较单纯照射组低平,但差异无统计学意义;给药+照射组的肿瘤控制率及肿瘤生长延迟远高于单独给药组及照射组(t=3.15~3.59,P＜0.05)。另外,氯氧喹并未增加辐射对小鼠血液细胞成分的不良反应。结论 氯氧喹对Lewis肺癌细胞及荷瘤小鼠均具有明显的辐射增敏作用,对小鼠血液系统未见明显不良反应,值得进一步深入研究。 |
| 英文摘要: |
| Objective To study whether Chloroxoquinoline could enhance the radiation sensitivity of Lewis lung cancer cells and xenograft tumors in tumor-bearing mice. Methods Both cell and animal experiments were divided into control group, drug group, irradiation group and combination group. MTT assay was used to detect the optical density of Lewis lung cancer cells cultured in vitro. The model of tumor-bearing mice was established first and then the tumor diameters of different groups were measured to figure out the tumor control rates and tumor growth delay. After irradiation, the blood samples in each group were collected to detect the amount of blood cells. Results MTT showed 24 hours after irradiation, the cell survival fraction of combination group was lower than that of irradiation group, but no statistical differences (P>0.05). However, 48 hours later, the cell survival fraction of combination group was obviously lower than irradiation group with statistically significant differences(t=3.22,4.23,4.46,4.58, P＜0.05). Animal experiments showed the tumor growth curve of combination group was significantly lower than irradiation group. The tumor control rate and tumor growth delay of combination group was more above the other groups(t=3.15-3.59,P＜0.05). Additionally, according to the analysis of blood samples, the hematological toxicity of chloroxoquinoline on mice was not found. Conclusion Chloroxoquinoline shows its radiation sensitizing effect on Lewis lung cancer cells and xenograft tumors in mice without hematological toxicity. |
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