| 乔俏,赵欣宇,李光.电离辐射诱导NF-κB-HIF-1α通路在人淋巴瘤细胞中的活化[J].中华放射医学与防护杂志,2011,31(1):25-28 |
| 电离辐射诱导NF-κB-HIF-1α通路在人淋巴瘤细胞中的活化 |
| Activation of NF-κB-HIF-1α pathway induced by X-ray irradiation in human lymphoma cells |
| 投稿时间：2010-02-28 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.007 |
| 中文关键词: 淋巴瘤 NF-κB HIF-1α 电离辐射 |
| 英文关键词:Lymphoma NF-κB HIF-1α Ionizing radiation |
| 基金项目:辽宁省教育厅基金(2009A754) |
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| 摘要点击次数: 3848 |
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| 中文摘要: |
| 目的 观察电离辐射对恶性淋巴瘤细胞中NF-κB-HIF-1α通路的活化状态的影响,探讨恶性淋巴瘤放射抗拒的可能机制。方法 采用Western blot免疫印迹分析方法,检测5 Gy X射线照射1、4、10和20 h后,Namalwa、Ramos和Raji 3种恶性淋巴瘤细胞系中NF-κB、HIF-1α及其相关蛋白IKK的表达水平,观察1、10和50 nmol/L NF-κB抑制剂QNZ对HIF-1α等蛋白表达的影响。结果 照射4 h后,3种淋巴瘤细胞中IKK蛋白表达增加(t=8.01、5.14和5.42,P<0.01)。10 h后,3种淋巴瘤细胞核p-P65蛋白表达增加(t=11.25、17.43和22.09, P<0.01),至20 h时表达开始下降。10~20 h时,3种淋巴瘤细胞HIF-1α蛋白表达增加,与0 h相比,差异均有统计学意义(t=19.05、12.44和12.88,P<0.01)。与单纯照射组相比,采用NF-κB抑制剂预处理3种肿瘤细胞24 h后, HIF-1α蛋白表达下降,且随药物浓度的增加而下降(t=18.69、19.35和12.26,P<0.01)。结论 电离辐射能够活化恶性淋巴瘤细胞中的NF-κB-HIF-1α通路,可能是肿瘤放射抗拒的机制之一。 |
| 英文摘要: |
| Objective To observe the effect of X-ray radiation on NF-κB-HIF-1α pathway activation in three malignant lymphoma and to explore the mechanism of radioresistance mediated by this pathway. Methods Expression levels of p-P65, IKK and HIF-1α in Namalwa, Ramos and Raji cells were determined by Western blot 1,4,10 and 20 h after 5 Gy X-ray irradiation. The effect of QNZ on HIF-1α expression was also observed. Results Expression level of IKK protein was up-regulated 4 h after irradiation in the 3 lymphoma cell strains(t=8.01, 5.14, 5.42, P<0.01), followed by up-regulation of p-P65 protein expression level at 10 h and HIF-1α at 10-20 h after X-ray irradiation (t=11.25, 17.43, 22.09, P<0.01). Pretreatment of QNZ 24 h before X-ray irradiation significantly reduced the up-regulation of HIF-1α protein expression induced by simple ion radiation (t=18.69, 19.35, 12.26,P<0.01). Conclusion NF-κB - HIF-1α pathway was activated in human lymphoma cells after ion radiation and may be involved in the mechanism of radioresistance. |
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