| 孙秀锦,崔凤梅,黄铖铖,等.60Co γ射线照射致小鼠肝脏的miRNAs表达改变[J].中华放射医学与防护杂志,2011,31(1):13-16.SUN Xiu-jin,CUI Feng-mei,HUANG Cheng-cheng,et al.Differential expression profiles of microRNAs in liver of 60Co γ-ray irradiated mice[J].Chin J Radiol Med Prot,2011,31(1):13-16 |
| 60Co γ射线照射致小鼠肝脏的miRNAs表达改变 |
| Differential expression profiles of microRNAs in liver of 60Co γ-ray irradiated mice |
| 投稿时间：2010-07-13 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.004 |
| 中文关键词: γ射线 照射 miRNA 芯片 生物信息学方法 |
| 英文关键词:γ-rays Irradiation miRNA Microarray Bioinformatic analysis |
| 基金项目:国家自然科学基金(30800922);江苏省高校自然科学研究项目(08KJD310007);2009年教育部"长江学者和创新团队发展计划资助"项目(IRT0849) |
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| 中文摘要: |
| 目的 应用miRNA芯片筛选4 Gy 60Co γ射线照射后小鼠肝脏中差异表达的miRNAs,生物信息学方法探索差异表达miRNAs调控的主要功能。方法 SPF级C57BL/6J小鼠接受4 Gy 60Co γ射线单次全身照射后,进行外周血白细胞计数和骨髓嗜多染红细胞微核计数。应用miRNA芯片筛选照射后小鼠肝脏中差异表达的miRNAs,用miRNA特异引物对部分差异表达miRNA进行实时定量PCR(real time PCR)验证。运用生物信息学方法,对差异miRNAs靶基因及调控功能进行预测。结果 4 Gy γ射线照射后,外周血白细胞总数与对照组相比显著减少(t=2.87,P<0.05),而骨髓嗜多染红细胞微核率与对照组相比显著增加(t=-2.91,P<0.05)。miRNA芯片结果显示,照射组与对照组差异表达的miRNAs共17个,其中9个表达上调,8个表达下调。miR-124和miR-34a的实时荧光定量RT-PCR验证结果与芯片结果一致。GO分析发现,与黏附、细胞周期相关的通路被抑制,一些免疫相关通路被激活。结论 miR-34a和miR-194参与了急性辐射损伤的调控,起主要调控作用的miRNAs还有miR-124、miR-382和miR-92a*。 |
| 英文摘要: |
| Objective To investigate the differential expression profiles of microRNAs in the liver of 60Co γ-ray irradiated mice using microRNA microarray and to explore their main functions by bioinformatic analysis. Methods After SPF C57BL/6J mice expose to 4 Gy-single whole body radiation,total number of peripheral WBC and the fMNPCE were measured at 3 d. The differentially expressed miRNAs in mouse liver were detected with miRNA microarray. miRNA-124 and miR-34a were confirmed by real time RT-PCR assay. Bioinformatic analysis was applied to explore target genes and the main functions of the differential expressed miRNAs. Results Compared with control group, the total number of peripheral WBC decreased(t=2.87, P<0.05), while the fMNPCE in bone marrow increased(t=-2.91, P<0.05) after 4 Gy γ-ray irradiation. miRNA microarray revealed that 17 miRNAs were differentially expressed, in which 9 up-regulated, 8 down-regulated. The expression levels of miR-124 and miR-34a were coincident with the result of real time RT-PCR.GO analysis showed that some pathways including adherens junction and cell cycle were suppressed, while some immune-related pathways were activated. Conclusions miR-34a and miR-194 were involved in the regulation of acute radiation damage,some other miRNAs including miR-124、miR-382 and miR-92a* also played important roles in radiation process. |
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